【摘 要】
：
The feeding method of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydro xyvalerate) [P(3HB-co-3HV)] by fed-batch culture of Ralstonia eutropha
【机 构】
：
Department of Chemical and Biomolecular Engineering,Department of Chemical Engineering
论文部分内容阅读
The feeding method of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydro xyvalerate) [P(3HB-co-3HV)] by fed-batch culture of Ralstonia eutropha was optimized to achieve high cell density and high 3HV yield. Effects of different feeding strategies of propionic acid on the production of P(3HB-co-3HV) were investigated. A decline of specific synthesis rate of copolymer and the yield of 3HV unit from propionic acid were observed due to the propionic acid accumulation in culture broth when the feeding solution with high P/G(propionic acid to glucose) ratio was employed. It was further confirmed by controlling propionic acid concentration at a low level in the separate feeding of propionic acid. An optimal feeding strategy was demonstrated to reduce the propionic acid accumulation. The cell concentration, P(3HB-co-3HV) productivity and 3HV unit fraction reached to 163.9 kg.m-3,1.8 kg.m-3.h-1, and 10.6%(by mass), respectively, resulting in a yield of 0.33 g HV per g propionic acid.
其他文献
Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses.In this study,
Soil salinization and non-point source pollution are among the most important and widespread environmental problems in European Mediterranean regions.Sweet sorg
Background Pulmonary capillary hemangiomatosis (PCH) is a rare disease and no Chinese case has been reported yet.The disease is often misdiagnosed and its clini
Root proliferation can be stimulated in a heterogeneous nutrient patch; however,the functions of the root proliferation in the nutrient-rich soil patches are no
To reduce the nitrate leaching risk after potato (Solanum tuberosum L.) harvest and improve nitrogen fertilizer-use efficiency,a potato-cabbage double cropping
Application of plant growth-promoting rhizobacteria (PGPR) has been shown to increase legume growth and development under field and controlled environmental con
Background Bronchial carcinoids are rare malignant neuroendocrine neoplasms.Some issues regarding surgical treatment of bronchial carcinoids remain controversia
Anhidrotic ectodermal dysplasia (EDA) is a relatively rare congenital hereditary disease.Because of a reduced number of sweat glands,patients are unable to pers