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目的:研究PTEN在结直肠癌中的表达及其与结直肠癌病理特征的关系．方法:应用免疫组化SP法分别检测65例癌组织和癌旁组织及13例腺瘤组织PTEN蛋白的表达．结果:PTEN主要在细胞核或细胞质中表达,在结直肠癌组织中阳性表达率显著低于癌旁组织阳性表达率(56．92% vs 86．15％,P<0．01)．中、高分化腺癌阳性表达率显著高于低、未分化腺癌阳性表达率(75．76% vs 37．50％, P<0．01)．Dukes A、B期阳性表达率显著高于Dukes C、D期阳性表达率(73．33％vs 42．86％, P<0．05)．此外,PTEN的表达与淋巴转移有关(P<0．01),而与性别、年龄、同期的肿瘤大小无关．结论:PTEN在结直肠癌组织中的表达下调,其表达水平可作为反映结直肠癌进展和预后的生物学指标之一．
Objective: To investigate the expression of PTEN in colorectal cancer and its relationship with the pathological features of colorectal cancer. Methods: The expression of PTEN protein was detected by immunohistochemical SP method in 65 cases of cancer tissues and adjacent tissues and 13 cases of adenomas. Results: PTEN was mainly expressed in nucleus or cytoplasm. The positive expression rate of PTEN in colorectal cancer tissues was significantly lower than that in paracancerous tissues (56.92% vs 86.15%, P <0.01). The positive expression rate of moderately and well-differentiated adenocarcinoma was significantly higher than that of low and poorly differentiated adenocarcinoma (75.76% vs 37.50%, P <0.01). The positive expression rate of Dukes A and B was significantly higher than that of Dukes C and D (73.33% vs 42.86%, P <0.05). In addition, the expression of PTEN was correlated with lymphatic metastasis (P <0.01), but not with the gender, age and tumor size at the same period. Conclusions: The expression of PTEN is down-regulated in colorectal cancer tissues, and its expression level can be used as one of the biological indicators to reflect the progress and prognosis of colorectal cancer.