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本研究用金帅和斯巴丹（Ｓｐａｒｔａｎ）两个苹果品种的茎顶在离体条件下分别嫁接在山定子、Ｍ７、Ｍ９和Ｍ２６的茎或根上，并获得成功。这种苹果显微嫁接方法尚未见报导。茎顶长度为０．２、０．４和０．７毫米，砧木长度为０．５—１．２厘米。嫁接成活率因接穗大小、嫁接方式而变化，其成活率范围在５６．３％到８１．３％之间。从获得无毒苗和具有较高的成活率两方面考虑，带有２片叶原基的茎顶作接穗是适当的。 嫁接七周后接穗干重因砧木不同而表现明显差异，矮砧上的接穗干物质积累量最少，半矮砧次之，乔化砧最多。作者认为，这可以作为一种鉴定砧木生长型的新途径，应进一步扩大砧木类型和品种的试验研究。
In this study, stems and roots of two apple cultivars, Ganshuai and Spartan, were successfully grafted onto the stems or roots of M7, M9 and M26 in vitro respectively under in vitro conditions. This apple micrografting method has not been reported. The tops of stems are 0.2, 0.4 and 0.7 mm, and the rootstocks are 0.5-1.2 cm in length. Grafting survival rate due to scion size, grafting methods and change, the survival rate in the range of 56.3% to 81.3%. From the point of view of obtaining non-toxic vaccine and high survival rate, it is appropriate to use scion top with 2 leaf primordia as scion. After seven weeks of grafting, the dry weight of scion showed obvious difference due to different rootstocks. The accumulation of scion dry matter on dwarf anvil was the least, followed by semi-dwarf anvil and Qiao anvil. The authors believe that this can be used as a new way to identify the growth of rootstocks should be further expanded the type of rootstocks and varieties of experimental research.