论文部分内容阅读
测定来自全国不同寄主和地区的211个青枯病细菌(Pseudomonassolanacearum)菌株产生细菌素的能力,结果表明,有80％分属于不同小种和生物型的8个指示菌能产生细菌素,其中能产生细菌素的桑菌株占其总数的94.2％。 用7个拮抗作用强而稳定的菌株测定对211个供试菌株的拮抗作用,结果表明,不同菌株产生细菌素的能力和拮抗范围有差异。其中油橄榄菌株(OE-104)和桑菌株(MA-1、MA-701)拮抗能力最强,拮抗范围最广。不同小种、生物型及寄主来源的菌株间的桔抗作用大于来源相同菌株间的拮抗作用。此外,试验证明培养温度、培养基成分和培养时间对菌株产生抑制圈的大小有影响。这些结果对筛选青枯病细菌的产细菌素拮抗菌有参考价值。
The ability to produce bacteriocins from 211 strains of Pseudomonasolanacearum from different hosts and regions in the country was determined. Eighty percent of the eight indicator bacteria belonging to different races and biotypes were capable of producing bacteriocins, of which, The bacteriocin producing strains account for 94.2% of the total. Seven antagonistic strains were tested for their antagonism against 211 tested strains. The results showed that there were differences in the ability of different strains to produce bacteriocins and their antagonistic range. Olive strains (OE-104) and Sang strains (MA-1, MA-701) antagonistic ability of the strongest, the most widely antagonistic. The antagonistic effects of different races, biotypes and host strains were greater than those of the same strain. In addition, the experiment proved that the culture temperature, medium composition and culture time had an effect on the size of the inhibition circle of the strain. These results have a reference value for screening bacteriocin antagonistic bacteria of bacterial wilt disease.