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扫描电镜见正常早代体外培养二倍体细胞 SL_7细胞膜表面有许多微绒毛,在晚代则明显减少,代之而起是许多球状突起、细胞碎屑、赘生物,加入 CH_3的 SL_7晚代细胞膜表面微绒毛仍多而球状突起少。透射电镜见正常早、中、晚代细胞的有嵴线粒体、粗面内质网、溶酶体、空泡小体和胞核的变化,与文献报道 WI-38株同类细胞变化相似。随胞龄增加,有嵴线粒体、粗面内质网渐减,溶酶体、空泡小体变大、增多。CH_3组晚代 SL_7细胞的有嵴线粒体和粗面内质网较正常对照组细胞为多,溶酶体、空泡小体较少,它提示 CH_3有延缓细胞微细结构衰退和延缓细胞衰老的作用,为 CH_3临床效用提供分子细胞学的依据。
Scanning electron microscopy showed that normal early generation culture diploid cells SL_7 cell membrane surface has many microvilli in the late was significantly reduced, replaced by many globular protrusions, cell debris, neoplasms, adding CH_3 SL_7 cell membrane Surface microvilli and globular processes are still less. Transmission electron microscopy showed that normal, early and late cells had changes in mitochondria, rough endoplasmic reticulum, lysosomes, vacuoles and nuclei, which were similar to those reported in the literature. With age increasing, there are ridges of mitochondria, rough endoplasmic reticulum decreases, lysosomes, vacuoles, larger, increased. The number of mitochondria and rough endoplasmic reticulum in SL_7 cells in CH_3 group was more than that in normal control group, and there were less lysosomes and vacuoles in SL_7 cells. It suggested that CH_3 could delay the decline of cell ultrastructure and delay the cell senescence , Provide molecular cytology basis for the clinical utility of CH_3.