论文部分内容阅读
目的：以正常淋巴结印片和切片配对实验作Feulgen染色DNA含量检测，探讨印片和切片在Feulgen染色各步骤的时间差异。方法：1、切片采用改进的Feulgen染色；印爿Feulgen染色分两组，一级水解60min，另一组水解90min。2、采用上海交大研制的ICM-100细胞DNA图像分析系统进行DNA含量检测，检测数据经SPSS统计软件包分析。结果：所有被检测切片和印片均得出单一DNA含量峰值，经SPSS统计软件包统计分析，切片和印片所检出的平均DNA值、DI值、2℃、3～4℃无显著性差异，P值均大于0．05。结论：在DNA含量检测Feulgen染色过程中，水解时间在切片和印片中是不一样的。
OBJECTIVE: To detect the content of Feulgen-stained DNA in normal lymph node prints and slice paired experiments, and to explore the time difference between the printed and sliced sections at each step of Feulgen staining. Methods: 1, the slices were improved Feulgen staining; stained Feulgen staining in two groups, a hydrolysis 60min, the other group hydrolysis 90min. The DNA content of ICM-100 cell DNA image analysis system developed by Shanghai Jiaotong University was tested. The detection data was analyzed by SPSS statistical package. Results: The peak value of single DNA content was obtained in all the tested sections and prints. The average DNA and DI values detected by the SPSS statistical package and the sections and prints did not show significant difference at 3 ℃ and 4 ℃ Differences, P values were greater than 0.05. Conclusion: During the Feulgen staining of DNA content, the hydrolysis time is different between slices and prints.