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目的:研究磷酸化的细胞外信号调节激酶1/2(ERK1/2)在鼻息肉组织中的表达及其意义。方法:应用免疫组织化学研究磷酸化的ERK1/2在鼻息肉组织和正常中鼻甲黏膜的表达及定位。进一步用免疫印迹对磷酸化ERK1/2在鼻息肉组织的表达进行半定量分析。结果:免疫组织化学发现,磷酸化ERK1/2在鼻息肉表达显著高于正常对照组,主要定位于增生的上皮细胞、炎症细胞、腺上皮细胞和内皮细胞,且以胞核阳性为主。免疫印迹结果显示鼻息肉中磷酸化ERK1/2的表达显著高于正常中鼻甲黏膜,为其4.2倍。结论:研究提示磷酸化ERK1/2可能在鼻息肉的发生发展过程中起重要作用。
Objective: To investigate the expression of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1 / 2) in nasal polyps and its significance. Methods: The expression and localization of phosphorylated ERK1 / 2 in nasal polyps tissue and normal middle turbinate mucosa were studied by immunohistochemistry. The expression of phosphorylated ERK1 / 2 in nasal polyp was further semi-quantitatively analyzed by immunoblotting. Results: Immunohistochemistry showed that the expression of phosphorylated ERK1 / 2 in nasal polyps was significantly higher than that in the normal control group, mainly located in hyperplastic epithelial cells, inflammatory cells, glandular epithelial cells and endothelial cells. Western blot results showed that the expression of phosphorylated ERK1 / 2 in nasal polyps was significantly higher than that of normal middle turbinate mucosa, which was 4.2 times. Conclusion: The results suggest that phosphorylation of ERK1 / 2 may play an important role in the development of nasal polyps.