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目的探讨重组人改构体酸性成纤维细胞生长因子(Mrh-aFGF)对帕金森病(PD)大鼠旋转行为和酪氨酸羟化酶(TH)免疫阳性神经元的影响。方法6-OHDA分别注入黑质和腹侧被盖区后建立PD大鼠模型,侧脑室内注射Mrh-aFGF,用阿扑吗啡诱导旋转行为,免疫细胞化学染色观察TH免疫阳性神经元和纤维,并进行定量分析。结果对照组均未引出旋转行为;PD组术后旋转启动时间缩短,持续时间延长,速度加快;生理盐水(NS)处理组旋转行为未见明显改善;Mrh-aFGF处理组旋转启动时间延长,持续时间缩短,速度减慢(P<0.01)。各组大鼠健侧黑质TH阳性神经元的数量维持在相近的水平。同组内健侧和损毁侧阳性神经元比较,对照组损毁侧无明显改变;PD组、NS处理组和Mrh-aFGF处理组损毁侧阳性神经元与健侧比较均明显减少(P<0.01)。其中PD组损毁侧黑质TH免疫阳性神经元数量随时间延长逐渐减少(P<0.01)。NS处理组损毁侧黑质TH免疫阳性神经元的变化与PD组相似;Mrh-aFGF处理组损毁侧黑质阳性神经元较PD组及NS处理组有明显改善,阳性神经元的数量明显增加(P<0.01)。结论Mrh-aFGF能减少PD大鼠黑质TH免疫阳性神经元的丢失,并改善其旋转行为。
Objective To investigate the effect of recombinant human recombinant human fibroblast growth factor (Mrh-aFGF) on the rotation behavior and tyrosine hydroxylase (TH) immunoreactive neurons in Parkinson’s disease (PD) rats. METHODS: PD rat model was established by injection of 6-OHDA into the substantia nigra and ventral tegmental area. Mrh-aFGF was injected into the lateral ventricle and apomorphine was used to induce rotational behavior. TH-immunoreactive neurons and fibers were observed by immunocytochemistry. And quantitative analysis. Results No rotation was induced in the control group. The rotational initiation time was shortened, the duration was prolonged and the speed was increased in the PD group. No significant improvement was observed in the rotation behavior in the NS group. The rotation start-up time in Mrh-aFGF treatment group was prolonged Time shortened, slowing down (P <0.01). The number of TH positive neurons in the substantia nigra in each group was maintained at similar levels. Compared with the contralateral and destructive neurons in the same group, there was no significant change in the damaged side of the control group; the lesion side and the contralateral side of PD group, NS group and Mrh-aFGF group were significantly decreased (P <0.01) . The number of TH-immunoreactive neurons in the lesioned side of PD gradually decreased with time (P <0.01). Compared with PD group, the change of nigral TH immunoreactive neurons in damaged side of NS group was similar to that in PD group; the number of substantia nigra neurons in injured group was significantly improved and the number of positive neurons in damaged group was significantly higher than that in PD group and NS group in Mrh-aFGF treatment group P <0.01). Conclusion Mrh-aFGF can reduce the loss of TH immunoreactive neurons in substantia nigra of PD rats and improve their rotation behavior.