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目的对深圳市2008年吸毒人群进行HIV-1分子流行病学调查研究,了解本地区吸毒人群HIV-1流行情况、亚型种类、毒株来源、变异情况等,为预防和控制HIV在吸毒人群中的流行提供有价值的资料。方法收集深圳市2008年份吸毒人群HIV-1抗体阳性样本21例,应用巢式聚合酶链式反应(nested-PCR)技术,对该样本膜蛋白基因(env基因)和核心蛋白(gag基因)进行扩增,并对其各基因区核苷酸序列进行测定和分析。结果21例HIV-1阳性样本中共存在CRF01-AE1种重组毒株以及A11种亚型,其在所有分析样本中的比例分别为90.5%和9.5%;其中12份Env基因样本中,10份为CRF01-AE重组亚型,与国际参考株01AE.TH.90.CM240最近,基因离散率为(9.910±2.432)%,组内离散率为(12.747±3.066)%;2份为A1亚型,与国际参考株a1.gu.92.92ug037最近,基因离散率为(17.15±0.354)%,组内基因离散率0.900%。21份Gag基因样本中,19份为CRF01-AE重组亚型,与国际参考株01AE.TH.90.CM240最近,基因离散率为(5.083±1.341)%,组内离散率为(6.072±1.968)%;2份A1亚型,与国际参考株01ae.cn.97.97cngx2f最近,基因离散率为(11.550±0.636)%,组内离散率为2.200%。结论2008年深圳地区HIV-1抗体阳性吸毒人群中HIV-1流行株以CRF01-AE重组亚型为主,并首次在深圳地区吸毒人群中发现A1亚型。
Objective To investigate the molecular epidemiology of HIV-1 among drug users in Shenzhen in 2008 to find out the HIV-1 prevalence, subtypes, origin and variation of HIV-1 in drug users in Shenzhen. To prevent and control HIV among drug users In the popular provide valuable information. Methods Totally 21 HIV-1 positive samples were collected from Shenzhen drug users in 2008, and the env gene and core protein (gag gene) of this sample were detected by nested-PCR. Amplified, and the nucleotide sequence of each gene was determined and analyzed. Results The CRF01-AE1 recombinant strains and A11 subtypes were co-existed in 21 HIV-1 positive samples, accounting for 90.5% and 9.5% of all the samples. Of the 12 Env samples, 10 were The CRF01-AE recombinant subtype, with the international reference strain 01AE.TH.90.CM240 recently, the gene dispersion rate was (9.910 ± 2.432)%, the intra-group dispersion rate was (12.747 ± 3.066)%; 2 copies of A1 subtype, With the international reference strain a1.gu.92.92ug037 recently, the genetic dispersion rate was (17.15 ± 0.354)%, within the group gene dispersion rate 0.900%. Of the 21 Gag gene samples, 19 were CRF01-AE recombinant subtypes, with the most recent genetic variation being (5.083 ± 1.341)% and (6.072 ± 1.968), respectively, compared with the international reference strain 01AE.TH.90.CM240 ); 2 subtypes A1, with the international reference strain 01ae.cn.97.97cngx2f recently, gene dispersion rate (11.550 ± 0.636)%, the intra-group dispersion rate of 2.200%. Conclusion In 2008, HIV-1 HIV-1 prevalence among HIV-1 positive drug users in Shenzhen was mainly CRF01-AE subtypes, and A1 subtypes were found in drug addicts in Shenzhen for the first time.