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目的为了解志贺菌毒力大质粒能否在体外转移及其转移后是否仍具有致病性。方法将人源福氏志贺菌及其毒力大质粒分别与鸡源鲍氏志贺菌混合,并在37℃培养箱和4℃冰箱中混合培养24 h;用不同剂量的毒力大质粒转移菌株通过口服和腹腔注射分别人工感染雏鸡和小白鼠。结果人源福氏志贺菌的毒力大质成功转移到丢失自身毒力大质粒的鸡源鲍氏志贺菌中;转移菌株对雏鸡和小白鼠均有致病性,且转移菌株的培养特性也有所改变。结论当志贺菌的毒力大质粒裸露出来且具有适宜的条件时,可以在体外转移到相近的病原菌中,且新生成的转移菌株具有一定稳定性和致病性;志贺菌的高致病性需要有毒力大质粒和菌体的其他特殊致病因子同时存在才能完成。
Objective To understand whether the virulence plasmid of Shigella could still be pathogenic after it was transferred in vitro and its metastasis. Methods The human Shigella flexneri and its virulence plasmids were respectively mixed with Shigella sonnei and were mixed in 37 ℃ incubator and 4 ℃ refrigerator for 24 h. Different doses of virulent plasmids Transfer strains were infected artificially with chicks and mice by oral and intraperitoneal injections, respectively. Results The virulence of human Shigella flexneri was successfully transferred to Shigella sonnei of chicken origin which lost its own virulence plasmid. The metastatic strain was pathogenic to chickens and mice, and the culture of the metastatic strain Features have also changed. Conclusion When the virulence plasmid of Shigella is exposed and has suitable conditions, it can be transferred to similar pathogens in vitro, and the newly generated metastatic strains have certain stability and pathogenicity. The high level of Shigella Pathogenic virions require large plasmids and other special virulence factors exist in order to complete.