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目的 观察霍乱毒素(cholera toxin,CT)佐剂和弓形虫排泄-分泌抗原(ESA)鼻内免疫小鼠诱导的抗弓形虫感染作用。方法 6周龄BALB/c小鼠60只,随机分为3组,每组20只。分别用PBS 20μl、ESA 20μg或CT 1.0μg+ESA 20μg每只滴鼻免疫2次,间隔2周。末次免疫后14 d,用4×104个弓形虫速殖子每只灌胃攻击所有小鼠,观察小鼠健康及死亡情况。速殖子攻击后30 d,计数肝、脑组织内弓形虫速殖子。结果 CT作为佐剂联合弓形虫ESA滴鼻免疫小鼠的健康状况明显好于PBS组和ESA组,存活率(95%)也显著高于PBS组(55%)。与PBS组相比,CT+ESA组肝和脑组织内速殖子数分别减少了80.19%(P<0.001)和78.24%(P<0.005)。CT作为佐剂联合ESA滴鼻免疫小鼠诱导了高水平的黏膜免疫应答和系统免疫应答。结论 CT作为佐剂联合弓形虫ESA滴鼻免疫小鼠诱导的免疫应答可有效抵抗弓形虫速殖子攻击。
Objective To observe the anti-Toxoplasma infection induced by intranasal immunization with cholera toxin (CT) adjuvant and Toxoplasma gondii-secreting antigen (ESA). Methods Sixty BALB / c mice aged 6 weeks were randomly divided into 3 groups (20 in each group). Each group was immunized twice with PBS 20 μl, ESA 20 μg or CT 1.0 μg + ESA 20 μg intranasally at intervals of 2 weeks. At 14 days after the last immunization, all the mice were challenged with 4 × 104 Toxoplasma tachyzoites to observe the health and mortality of mice. Thirty days after tachyzoite challenge, Toxoplasma tachyzoites in the liver and brain tissues were counted. Results The immune status of mice immunized with ESA as adjuvant and ESA was better than that of PBS group and ESA group. The survival rate (95%) was also significantly higher than that of PBS group (55%). Compared with the PBS group, tachyzoites in the liver and brain of CT + ESA group were reduced by 80.19% (P <0.001) and 78.24% (P <0.005), respectively. Immunization of mice intranasally with CT as an adjuvant in combination with ESA induced a high level of mucosal and systemic immune responses. Conclusion CT as an adjuvant combined with Toxoplasma gondii ESA intranasal immunization of mice induced immune response can effectively resist Toxoplasma gondii attack.