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目的探讨荧光定量聚合酶链反应（简称 ＦＱ－ＰＣＲ）检测 ＨＢＶ－ＤＮＡ的临床意义。方法对 １１５例乙型肝炎患者的血清同时进行乙肝两对半、ＡＬＴ、ＡＳＴ及ＨＢＶ－ＤＮＡ测定。结果大三阳患者血清ＨＢＶ－ＤＮＡ阳性率为 １００％，平均含量为 １０７．２３±１．６４拷贝／ｍｌ；小三阳患者血清 ＨＢＶ－ＤＮＡ阳性率为 ５９．６％，平均含量为 １０６．２１±１．５１拷贝／ｍｌ；两组之间的 ＨＢＶ－ ＤＮＡ含量差异有显著性。乙肝两对半全阴性 ２２例，有 ６例 ＨＢＶ－ ＤＮＡ阳性，其平均浓度为１０４．１２±１．３８拷贝／ｍｌ与大、小三阳患者的浓度差异有非常显著性。结论ＦＱ－ＰＣＲ法检测乙肝患者血清ＨＢＶ－ＤＮＡ具有快速、特异、灵敏等优点，它对乙型肝炎的诊断、治疗、预防具有重要的临床意义。
Objective To investigate the clinical significance of detecting HBV-DNA by fluorescence quantitative polymerase chain reaction (FQ-PCR). Methods Two hundred and seventy hepatitis B patients with ALT, AST and HBV-DNA were detected in 115 patients with hepatitis B at the same time. Results The positive rate of serum HBV-DNA in patients with Dasan Yang was 100% with an average of 107.23 ± 1.64 copies / ml. The positive rate of HBV-DNA in patients with Sansanying was 59.6% with an average of 106.21 ± 1.51 copies / ml; HBV-DNA content between the two groups was significantly different. Hepatitis B two pairs of semi-negative in 22 cases, 6 cases of HBV-DNA positive, the average concentration of 104.12 ± 1.38 copies / ml and large and small three positive patients with significant differences in concentration. Conclusion The detection of serum HBV-DNA in patients with hepatitis B by FQ-PCR is rapid, specific and sensitive. It has important clinical significance for the diagnosis, treatment and prevention of hepatitis B.