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本研究利用Charleston×东农594得到的147个F2:14-F2:19重组自交系群体,对11个环境条件下大豆荚数性状相关QTL的加性、上位性及其与环境互作效应进行了分析。在6年11个不同遗传背景条件下的多环境联合分析中定位了11个QTL具有加性效应,其加性(A)贡献率和AE互作贡献率都是微效的。联合分析同时定位到20对QTL具有上位效应,并发现上位QTL的2种作用模式,一种是同一连锁群上2个QTL间的上位性互作,另一种是不同连锁群上2个QTL间的上位性互作。鉴定出9个具有加性效应的QTL能在多个环境条件下被检测到,17对具有上位性效应的QTL能在多个环境条件下被检测到,部分QTL的上位性效应解释的表型变异大于5%。这些在不同环境或不同遗传背景下检测到的QTL,可作为大豆荚数相关性状改良的候选标记,用于分子标记辅助选择或图位克隆。
In this study, 147 F2: 14-F2: 19 recombinant inbred lines obtained from Charleston × Dongnong 594 were used to study the additive and epistatic QTLs for soybean pod character under 11 environmental conditions and their interactions with environment Analyzed. Eleven QTLs were additive in the multi-environment joint analysis of eleven different genetic backgrounds in six years. The contribution of the additive (A) contribution and the contribution of AE interaction were all marginal. The joint analysis identified 20 epistatic effects of QTL at the same time and found two modes of action of the higher QTLs: one is the epistatic interaction between two QTLs on the same linkage group, and the other is two QTLs on different linkage groups Between the epistatic interaction. Nine QTLs with additive effects were identified to be detected under a variety of environmental conditions. Seventeen QTLs with epistatic effects were detected under several environmental conditions. The phenotypes explained by epistasis effects of some QTLs Variation greater than 5%. These QTLs detected in different environments or in different genetic backgrounds can be used as candidate markers for the improvement of soybean pod-related traits for molecular marker-assisted selection or map cloning.