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目的探讨重庆地区住院毛细支气管炎患儿鼻咽分泌物中HBoV和其他10种呼吸道病毒检出率及其流行特征变化。方法收集2006-2008年的毛细支气管炎发病高峰季节(2006年11月至2007年3月和2007年11月至2008年3月)住院毛细支气管炎患儿的鼻咽分泌物,首先通过直接免疫荧光进行呼吸道常见7种病毒筛查,然后提取鼻咽分泌物病毒的DNA和RNA,用PCR/RT-PCR的方法检测HBoV等其余10种常见呼吸道病毒相应基因片段。结果①两时间段患儿的年龄比较,差异具有显著性(P<0.01)。临床症状中,发热、呕吐、气促的发生例数差异具有显著性(P<0.05)。其余情况差异均不显著;②重庆地区住院毛细支气管炎患儿鼻咽分泌物中共检出十余种呼吸道病毒,112例患儿呼吸道标本中共检出呼吸道病毒阳性84例(75%),其中单一病毒阳性45例(40.18%),2种或2种以上病毒合并阳性39例(34.82%),2006年11月至2007年3月和2007年11月至2008年3月毛细支气管炎发病高峰季节毛细支气管炎患儿病毒检出情况不同;③112例患儿中最常见的为RSV,其余依次为PIV3,RV等,检出HBoV10例,均为合并感染。结论①毛细支气管炎患儿鼻咽分泌物呼吸道病毒感染阳性检出率高,病毒种类复杂,合并感染率高。而且每年的病毒检出情况、合并感染情况、流行季节变化可能与不同年份患儿临床特征不同有关;②在住院毛细支气管炎患儿鼻咽分泌中新近发现HBoV感染,以合并感染为主,但其检出阳性率明显低于RSV和hMPV。
Objective To investigate the prevalence and prevalence of HBoV and other 10 respiratory viruses in nasopharyngeal secretions of children with hospitalized bronchiolitis in Chongqing area. Methods The nasopharyngeal secretions of children with bronchiolitis were collected during the peak season of bronchiolitis in 2006-2008 (from November 2006 to March 2007 and from November 2007 to March 2008). The nasopharyngeal secretions were collected by direct immunization Fluorescence screening of seven common respiratory viruses, and then extract nasopharyngeal secretions of the virus DNA and RNA, PCR / RT-PCR method to detect HBoV and other 10 kinds of common respiratory virus corresponding gene fragments. Results ① There was significant difference in age between two groups (P <0.01). Clinical symptoms, fever, vomiting, shortness of breath there were significant differences in the number of cases (P <0.05). The rest of the cases were not significant differences; ② Chongqing residents with bronchiolitis in children with nasopharyngeal secretions detected a total of more than 10 kinds of respiratory virus, 112 cases of respiratory tract specimens were detected positive in respiratory viruses in 84 cases (75%), of which a single 45 cases (40.18%) were positive for virus, 39 cases (34.82%) were positive for two or more viruses, the peak season of bronchiolitis occurred from November 2006 to March 2007 and from November 2007 to March 2008 Bronchiolitis in children with different detection of the situation; ③ 112 cases of children with the most common RSV, the rest followed by PIV3, RV, etc., were detected in 10 cases of HBoV, were co-infection. Conclusions ① The detection rate of nasopharyngeal secretions respiratory virus infection in children with bronchiolitis is high, the virus types are complex, and the infection rate is high. And the annual detection of the virus, the co-infection, the seasonal changes in prevalence may be different clinical features of children with different years; ② newly diagnosed nasopharyngeal secretion in children with hospitalized bronchiolitis HBoV infection, mainly in combination with infection, The positive rate was significantly lower than RSV and hMPV.