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目的:建立不同产地蜂胶的质量评价方法。方法:采用薄层色谱法,以白杨素和高良姜素为对照品,甲苯-乙酸乙酯-甲酸(10∶4∶3)为展开剂,进行定性鉴别研究;采用高效液相色谱法,色谱柱Dikma Technologies(250 mm×4.6mm,5μm);流动相甲醇-0.15%磷酸水溶液(62∶38);流速1.0 mL·min-1;柱温25℃;检测波长268 nm。结果:28个产地蜂胶原料药中白杨素和高良姜素的含量均符合《中国药典》(2010年版)的规定的仅有6个地区,大部分地区蜂胶原料药中高良姜素含量符合标准,而白杨素含量不达标。结论:不同产地蜂胶原料药中白杨素和高良姜素的含量不同,建立的定性、定量方法准确可靠,专属性强,适合不同产地蜂胶原料药的质量控制。
Objective: To establish the quality evaluation method of propolis in different areas. Methods: TLC and HPLC were used for the determination of chrysin and galangin. Toluene-ethyl acetate-formic acid (10: 4: 3) was used as the developing reagent. The compounds were identified by high performance liquid chromatography Column Dikma Technologies (250 mm × 4.6 mm, 5 μm); mobile phase methanol-0.15% phosphoric acid (62:38); flow rate 1.0 mL · min-1; Results: The content of chrysin and galangin in 28 producing propolis raw materials all met the requirements of “Chinese Pharmacopoeia” (2010 edition), only 6 areas. The content of galangin in most of the propolis raw materials met the standard, The content of chrysin is not up to standard. CONCLUSION: The contents of chrysin and albuterin in the propolis raw materials from different areas are different. The established qualitative and quantitative methods are accurate, reliable and specific, and are suitable for the quality control of propolis raw materials in different areas.