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目的:对子痫前期患者血清差异蛋白进行分析,并对差异蛋白的鉴定方式进行研究。方法:选择子痫前期患者20例及同期正常妊娠患者10例,应用表面增强激光解析电离飞行时间质谱技术对入选患者的血清蛋白进行检测,并获取蛋白质谱,分析差异蛋白;将差异蛋白的分子量代入蛋白质数据库中进行对比,查找与其分子量最为相近的蛋白质,鉴定差异蛋白种类。结果:轻度子痫前期患者与正常妊娠患者间的差异蛋白质荷比为3 936、3 263、13 773,P<0.05;轻度子痫前期与重度子痫前期患者间的差异蛋白质荷比为3 263、7 982、13 773、15 943,P<0.05;质荷比为3 263、13 773蛋白质的相对含量会随着病情的加重不断增加,P<0.05;差异蛋白分子量代入蛋白数据库对比后,相近分子量的蛋白名称为胰岛淀粉样多肽、血管活性肠肽、低诱导趋化因子A2前体、铁氧化还原样氮74个氨基酸、心房利钠因子前体。结论:分析并鉴定子痫前期患者的血清差异蛋白,可为子痫前期的早期诊断与预防提供诊疗依据,具有重要的临床应用价值。
Objective: To analyze the serum differential proteins in patients with preeclampsia and to identify the differential proteins. Methods: Twenty patients with preeclampsia and 10 normal pregnant women were enrolled in this study. Serum proteins of patients were detected by surface enhanced laser desorption / ionization time-of-flight mass spectrometry (GC-MS / MS), and protein profiles were analyzed to analyze the differences in protein molecular weight Into the protein database for comparison, find the most similar to the molecular weight of the protein, identification of different types of proteins. Results: The difference in protein-to-protein load ratio between mild preeclampsia and normal pregnancy was 3 936, 3263, 7373, P <0.05; the difference in protein to protein between mild preeclampsia and severe preeclampsia was 3 263,7 982,13 773,15 943, P <0.05; the mass-to-charge ratio was 3 263, the relative content of 7 773 protein increased with the increase of disease, P <0.05; , Similar molecular weight protein name is amylin, vasoactive intestinal peptide, low-induced chemokine A2 precursor, iron redox-like nitrogen 74 amino acids, atrial natriuretic factor precursor. Conclusion: Analyzing and identifying serum differential proteins in patients with preeclampsia may provide a basis for diagnosis and prevention of preeclampsia, and has important clinical value.