论文部分内容阅读
目的:证实链脲佐菌素(STZ)腹腔注射法建立大鼠糖尿病性勃起功能障碍(ED)模型的可行性。方法:60只SD大鼠随机分为对照组(10只)和试验组(50只)。试验组按照50mg/kg的剂量单侧腹腔注射即时配制的STZ溶液。对照组单侧腹腔注射同等量的柠檬酸-柠檬酸钠缓冲液0.1mol/L。第4天测血糖,以随机血糖>16.7mmol/L定为建模标准;平均每周监测血糖1次,10周后将阿朴吗啡(APO)按100μg/kg比例皮下注射于大鼠颈部皮肤松软处,观察大鼠阴茎勃起情况。结果:实验组糖尿病成模率为94%(47/50),有3只大鼠出现STZ抵抗,成模后10周相继有10只大鼠死亡;试验组血糖明显高于正常对照组(P<0.01),体重、阴茎勃起次数、阴茎勃起率则明显低于对照组(P<0.01)。结论:STZ 50mg/kg单侧腹腔内注射能安全有效地建立大鼠糖尿病模型,颈部皮下注射APO 100μg/kg可有效地筛选出大鼠糖尿病勃起功能障碍。
Objective: To confirm the feasibility of streptozotocin (STZ) intraperitoneal injection in establishing rat diabetic erectile dysfunction (ED) model. Methods: Sixty SD rats were randomly divided into control group (n = 10) and experimental group (n = 50). Experimental group according to the dose of 50mg / kg unilateral intraperitoneal injection of STZ solution. The control group received unilateral intraperitoneal injection of the same amount of citric acid-sodium citrate buffer 0.1 mol / L. On the fourth day, the blood glucose was measured, and the random blood glucose> 16.7mmol / L was taken as the modeling standard. The average blood glucose was monitored once a week. After 10 weeks, apomorphine (APO) was subcutaneously injected into the rat’s neck at a dose of 100μg / kg Soft skin, observe the penile erection of rats. Results: The incidence of diabetes mellitus in experimental group was 94% (47/50). STZ resistance was found in 3 rats. Ten rats died after 10 weeks after operation. The blood glucose in experimental group was significantly higher than that in normal control group (P <0.01). The body weight, erection frequency and penile erection rate were significantly lower than those in the control group (P <0.01). Conclusion: STZ 50mg / kg unilateral intraperitoneal injection can be safely and effectively established rat model of diabetes, subcutaneous injection of APO 100μg / kg in the neck can effectively filter out diabetic erectile dysfunction.