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目的研究核桃楸皮的抗衰老自由基的作用机制。方法健康成年ICR小鼠60只,雌雄各半,用四唑盐(MTT)微量自动比色法测定脾淋巴细胞转化指数(SI);用免疫荧光法测定脾T淋巴细胞亚群;用PI染色法测脾淋巴细胞的凋亡率,观察其对免疫功能的影响。结果 60只雄性大鼠除正常组外,实验组小鼠大脑组织匀浆液D值在15以上,(P<0.05)。结论通过对核桃楸皮的药理试验,说明核桃楸皮具有抗衰老的作用,为临床研制经济型、天然无毒抗自由基、抗衰老的新药研制提供依据。
Aim To study the anti-aging free radical mechanism of walnut. Methods Sixty adult ICR mice were randomly divided into male and female mice. The spleen lymphocyte transformation index (SI) was determined by MTT assay. The splenic T lymphocyte subsets were detected by immunofluorescence. The apoptosis rate of splenic lymphocytes was measured to observe its effect on immune function. Results In 60 male rats, except for the normal group, the D value of brain tissue homogenate in the experimental group was above 15 (P <0.05). Conclusion Through the pharmacological test on walnut tree, the walnut tree has anti-aging effect, which provides a basis for the clinical research of economical, natural non-toxic anti-free radical and anti-aging new drug.