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目的 研究 5 0Hz工频磁场对细胞膜表皮生长因子 (EGF)和肿瘤坏死因子 (TNF)受体聚簇的可能诱导作用 ,进一步说明工频磁场生物效应细胞信号起始部位及噪声磁场可能存在的干预作用。方法 将中国仓鼠肺成纤维细胞分别用EGF、TNF、0 4mT的工频磁场、噪声磁场或工频磁场与噪声磁场叠加的复合磁场处理一定时间后 ,以免疫组化方法标记细胞膜EGF及TNF受体 ,并用激光共聚焦显微镜观察分析细胞膜EGF及TNF受体的聚簇程度。结果 细胞经EGF及TNF处理 5min后即可诱导相应受体的聚簇 ,0 4mT工频磁场辐照 5min后也可诱导细胞膜EGF及TNF受体的聚簇 ,并均在 15min时达到最大程度。然而相同强度的噪声磁场不能诱导细胞膜受体的聚簇。当等强度的噪声磁场与工频磁场叠加后 ,可以抑制工频磁场诱导的细胞膜EGF及TNF受体聚簇。结论 5 0Hz工频磁场与相应配体一样 ,能诱导细胞膜EGF及TNF受体的聚簇 ,膜受体是电磁场可能的信号耦合位点之一。等强度的噪声磁场可干预工频磁场对细胞膜受体聚簇的诱导。
Objective To investigate the possible induction of EGF and TNF receptor clustering induced by 50 Hz power frequency magnetic field and to further explain the potential interference of signal magnetic field effect. Methods The Chinese hamster lung fibroblasts were treated with EGF, TNF, 0,4 mT frequency magnetic field, noise magnetic field or frequency magnetic field and noise magnetic field for a certain period of time, respectively, and the EGF and TNF receptors were labeled by immunohistochemistry Body, and confocal laser scanning microscopy analysis of cell membrane EGF and TNF receptor clustering degree. Results The cells were induced by EGF and TNF for 5 minutes, and the corresponding receptors were induced to cluster. After 4 minutes of 0 4 mT magnetic field irradiation, the EGF and TNF receptors were also induced to aggregate to the maximum at 15 minutes. However, the same intensity of noise magnetic field can not induce the clustering of cell membrane receptors. When the same intensity of noise magnetic field and power frequency magnetic field superposition, can inhibit the frequency of magnetic field induced cell membrane EGF and TNF receptor clustering. Conclusion The 50 Hz power frequency magnetic field, like the corresponding ligand, can induce the clustering of EGF and TNF receptors in the cell membrane. The membrane receptor is one of the potential signal coupling sites in the electromagnetic field. The equal strength of the noise magnetic field can intervene in the frequency magnetic field induced by the cell membrane receptor clustering.