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用5只单侧后肢备用根猫术后5d的两侧脊髓背角组织提取液进行聚丙烯酰肢凝胶电泳，再以鸡胚背根节种经细胞构蛋白带技术找寻有神经营养活性的蛋白带．结果显示：手术侧组的电泳凝胶条上相对迁移享0．10～0．13和0．43～0．50两处均有较多的神经细胞附着，细胞数分别是165个2.5×105μm2、216个／2．5×105μm2．非手术侧组凝胶条相应两处同一面积内仅有1或2个细胞．两侧相比有显著性差异（P＜0001），提示手术侧组电泳凝胶条相对迁移率0．10～013与043～0．50两处含有能维持种经元存活的神经营养活性物质．
Five unilateral hindlimb backup root cats on both sides of the spinal cord 5 d after the dorsal horn tissue extracts by polyacrylamide gel electrophoresis, and then the chicken embryo dorsal root segments through the cell structure protein band technology to find neurotrophic activity Protein band. The results showed that there were more neuronal cells attached at the two sides of 0.10 ~ 0.13 and 0.43 ~ 0.50 in the migration side of the electrophoresis strip on the operation side, respectively. The number of cells was 165 2.5 × 105μm2 , 216 /2.5 × 105 μm2. The non-surgical side of the gel strip corresponding to the same area of two only 1 or 2 cells. There was significant difference between the two sides (P <0.0001), suggesting that the relative mobility of gel electrophoresis in the lateral group of the operation group was between 0.10-013 and between 043 and 0.50, which contained the neurotrophic substances .