论文部分内容阅读
HCV为单股正链RNA病毒,基因结构与黄病毒相似,并具有高度异源性和显著变异性。HCV感染后,少部分表现为急性肝炎,大都表现为慢性迁延性肝炎、慢性活动性肝炎、肝硬化及肝细胞肝癌。慢性持续感染率达70％左右。研究丙型肝炎病毒的致病机制已成为世界肝脏疾病的研究重点。本研究课题首先选择HCV病毒5′—非编码区为靶基因区,在体外用RT—PCR方法有效地扩增该区基因,检测慢性非甲非乙型肝炎患者及肝细胞肝癌患者血清及肝组织中HCV—RNA;进而分析5′—非编码区及非结构区5的核苷酸变异;随之用基因工程重组α—干扰素(IFN)治疗已确诊为HCV感染的慢性肝炎患者,研究IFN对病毒复制的抑制,更深入地研究HCV病毒复制中间体负链RNA在肝内、外组织的分布定位,研究HCV对T细胞及B细胞的影响,同时建立了HCV相关肝癌细胞株,研究HCV对抑癌基团、癌基团的调控作用的影响。
HCV is a single-stranded positive-sense RNA virus with a gene structure similar to that of the flavivirus and is highly heterologous and notable for variability. After HCV infection, a small part of the performance of acute hepatitis, mostly manifested as chronic persistent hepatitis, chronic active hepatitis, cirrhosis and hepatocellular carcinoma. Chronic persistent infection rate of about 70%. Research on the pathogenesis of hepatitis C virus has become the focus of the world’s liver disease research. In this study, we first selected the 5 ’non-coding region of the HCV virus as the target gene region, in vitro amplification of this gene by RT-PCR method to detect the serum and liver of patients with chronic non-A, non-B hepatitis and hepatocellular carcinoma HCV-RNA in tissues; further analysis of nucleotide variations in 5’-noncoding and non-structural regions 5; followed by treatment of patients with chronic hepatitis C that have been diagnosed with HCV infection with genetically engineered recombinant alpha interferon (IFN) IFN inhibition of viral replication, and more in-depth study of HCV viral replication intermediate negative strand RNA distribution in the liver and outside the organization to study the impact of HCV on T cells and B cells, while the establishment of HCV-related liver cancer cell lines, research Effect of HCV on the regulation of tumor suppressor and oncogene.