Fas与体外胸腺细胞自发凋亡的关系

• 作 者: 蒋定文 郭明秋 陈立茵 沈先荣 丁丽
• 机 构: 上海海军医学研究所!上海市翔殷路 880号邮编: 200433,第一军医大学基础部抗衰老研究室!广东广州 510515,第一军医大学基础部抗衰老研究室!广东广州 510515,上海海军医学研究所!上
• 出 处: 细胞与分子免疫学杂志
• 发表日期: 2001年01期
• 关键词: 胸腺细胞 Fas 体外培养 胸腺微环境 细胞调亡 胸腺基质细胞 细胞分化 免疫应答 台盼蓝染色 流式细胞术

目的探讨体外培养的胸腺细胞表达Ｆａｓ、Ｆａｓ－Ｌ与自发凋亡的关系。方法应用流式细胞术，检测体外培养不同时间的胸腺细胞的凋亡率及Ｆａｓ、Ｆａｓ－Ｌ表达的阳性率。结果体外培养的胸腺细胞自发凋亡率和Ｆａｓ表达阳性率，均呈时间依赖性增加， Ｆａｓ－Ｌ在体外培养２４ ｈ时明显增加。体外培养的胸腺细胞Ｆａｓ的表达与自发凋亡明显相关。结论Ｆａｓ是介导体外培养的胸腺细胞自发凋亡的重要分子。

Objective To investigate the relationship between the expression of Fas, Fas-L and the spontaneous apoptosis in cultured thymocytes. Methods Flow cytometry was used to detect the apoptotic rate of thymocytes and the positive rate of Fas and Fas-L expression in vitro. Results The apoptotic rate of thymocytes and the positive rate of Fas expression in vitro were increased in a time-dependent manner. Fas-L increased significantly after cultured in vitro for 24 h. The expression of Fas in thymocytes cultured in vitro was significantly associated with spontaneous apoptosis. Conclusion Fas is an important molecule that mediates spontaneous apoptosis of thymocytes cultured in vitro.