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首次采用特殊方法从感染兔红细胞上分离、纯化两种血吸虫组分抗原SjR47和SjR12，实验结果证明，前者属卵源性抗原，后者为虫源性抗原．两次实验结果均表明，两种组分抗原能诱导出明显的抗病免疫力，实验1获得54．95％－72．36％的肝内减卵率，实验2获得52．57％－57.27％的肝内减卵率和50.73％－74．70％的成熟卵减少率，并均可使肝内肉芽肿病变明显减小，尤以SjR47效果显著．两种抗原诱导的抗肝内肉芽肿保护性免疫调节机制的研究结果表明，两者均能诱导小鼠产生高水平的IgG，测定IgG及其亚类动态显示，在感染后0－12wk时，免疫小鼠血清IgG和IgG_1水平均显著高于佐剂和感染对照组者（P＜0.01），特别是能反映保护性免疫力指标的IgG_1升高非常明显：SjR47免疫鼠的脾T淋巴细胞亚群表型动态研究显示，SjR47免疫鼠的CD4~＋和CD8~＋T细胞在感染4wk时均升高，随后下降．CD4~＋／CD8~＋比值也随时间延长而下降，呈现免疫下调，其变化趋势同肉芽肿病变减小相一致；SjR47免疫鼠的细胞因子水平动态观察结果表明，SjR47免疫鼠后可使TNF－α在小鼠体内的活性水平较平显著上升（成虫期前），减弱了TNF－α对虫卵肉芽肿形成的介导作用以及对雌虫排卵的促进作用，导致肝内肉芽肿病变减小和虫卵减少。本研究结果表明，两
For the first time, a special method was used to separate and purify the two kinds of antigenic components SjR47 and SjR12 of rabbit erythrocytes. The experimental results show that the former belongs to egg-derived antigen and the latter is insect-derived antigen. The results of the two experiments showed that two components of the antigens can induce significant resistance to immunity, the experiment 1 54.00% -72.36% intrahepatic egg reduction rate, experiment 2 52.57% -57.27 % Of intrahepatic egg reduction and 50.73% -74.70% of mature egg reduction rates, and can significantly reduce intrahepatic granuloma lesions, especially SjR47 significant effect. Two antigen-induced anti-intrahepatic granuloma protective immune regulatory mechanism of the results showed that both can induce high levels of IgG produced in mice, IgG and its sub-class dynamic showed that at 0-12wk infection, The level of serum IgG and IgG_1 in the immunized mice were significantly higher than that in the adjuvant and control group (P <0.01). Especially, IgG1, which can reflect the indicators of protective immunity, was obviously increased: the spleen T lymphocyte subsets of SjR47 immunized mice The study of population phenotype showed that the CD4 ~ + and CD8 ~ + T cells of SjR47 immunized mice both increased 4wk and then decreased. The ratio of CD4 ~ + / CD8 ~ + also decreased with time, showing immunoregulation, the trend of which was consistent with the decrease of granuloma lesion. The dynamic observation of the cytokine levels of SjR47 immunized mice showed that TNF- -α activity in mice increased significantly (pre-adult stage), attenuated the mediated effect of TNF-α on the formation of ovarian granuloma and promoted the ovulation in females, resulting in the decrease of intrahepatic granuloma Small and reduced eggs. The results of this study show that two