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从欧洲中部及美国收集了 18个刺槐种源种子 ,以 2年生苗木为材料 ,采用水平切片淀粉凝胶电泳和聚丙烯酰胺凝胶电泳对 11个酶系统进行了检测 ,共发现 2 0个酶位点 ,其中 14个为多态位点 ,多态性位点百分数为70 %。在参加计算的 7个酶系统的 12个多态性位点中 ,平均每个位点的等位基因数 (A)和平均每位点等位基因有效数 (Ae)为 2 733和 1.794 ,平均每个位点的实际杂合度 (Ho)和预期杂合度 (He)为 0 36 8和 0 4 0 0 ,固定指数 (F)为 0 0 80 ,绝大多数位点固定指数为正值。各基因位点遗传参数与 12个位点平均值进行相关分析表明 ,Fe b和Lap a的大部分群体遗传参数 (A、Ae、Ho、He等 )与平均值存在紧密的相关关系 ,其他位点各项遗传参数和总平均值相关不明显 ,这些位点的重要性可能比相关性较好的位点更大。德国 8个种源群体间遗传距离变化在 0 0 9～0 2 6之间 ,来自匈牙利的 6个种源之间遗传距离很小 ,绝大部分均在 0 11以下 ;德国的 8个种源与匈牙利和斯洛伐克的 8个种源之间的遗传距离变化在 0 0 9～ 0 2 4之间 ;来自美国的 2个种源 ,与欧洲的种源间的遗传距离在0 0 9～ 0 2 3之间 ,这一距离没有超出欧洲种源之间的差异。匈牙利和斯洛伐克的 8个种源群体的各项遗传参数均高于德国种源 ,表明这?
Eighteen Robinia pseudoacacia seeds were collected from central Europe and the United States. Two-year-old seedlings were used as materials. Eleven enzyme systems were detected by horizontal sectioned starch gel electrophoresis and polyacrylamide gel electrophoresis. A total of 20 enzymes Among them, 14 were polymorphic and the percentage of polymorphic loci was 70%. The average number of alleles per locus (A) and the average number of alleles per locus (Ae) were 2 733 and 1.794 among the 12 polymorphic sites involved in the calculation of the seven enzyme systems, The average heterozygosity (Ho) and expected heterozygosity (He) of each locus were 0 36 8 and 0 40 0, the fixed index (F) was 0 0 80, and the majority of locus fixed index was positive. Correlation analysis between the genetic parameters of each locus and the average of 12 loci showed that most of the population genetic parameters (A, Ae, Ho, He, etc.) of Fe b and Lap a were closely related to the mean value. Point genetic parameters and the overall average correlation is not obvious, these sites may be more important than the relevance of the larger sites. The genetic distances among 8 provenances in Germany ranged from 0 0 9 to 0 26, and the genetic distances among 6 provenances from Hungary were very small, the majority of them were below 0 11. Eight provenances from Germany The genetic distance to eight provenances in Hungary and Slovakia varied from 0 0 9 to 0 2 4. The genetic distances from 2 provenances in the United States to those in Europe ranged from 0 0 9 to 0 2 3, this distance does not exceed the difference between European provenances. The genetic parameters of the eight provenance groups in Hungary and Slovakia are higher than those in Germany, indicating that?