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目的探讨地塞米松(Dexamethasone,Dex)对脂多糖(Lipopolysaccharide,LPS)诱导的急性肺损伤(acute lung injury,ALI)小鼠肺组织中髓样细胞表达的触发受体1(triggering receptor expressed on myeloid cells-1,TREM-1)表达的影响。方法以昆明小鼠为研究对象,腹腔注射LPS(10 mg/kg)建立ALI模型,30 min后给予不同浓度的Dex(5、10、20、40 mg/kg)处理6 h;选用Dex(10 mg/kg)处理不同的时间(6、12、24、36 h)。HE染色法观察肺组织病理损伤程度;RT-PCR检测肺组织TREM-1mRNA的表达;ELISA检测小鼠支气管肺泡灌洗液中可溶性TREM-1(s TREM-1)蛋白水平。结果 Dex可减轻肺病理损伤;Dex呈时间依赖性地下调ALI小鼠肺组织的TREM-1 mRNA的表达,且在6 h即可降低;Dex呈剂量依赖地下调ALI小鼠肺组织中TREM-1 mRNA的表达,并在10 mg/kg时开始降低。Dex可降低ALI小鼠支气管肺泡灌洗液中sTREM-1的蛋白水平。结论 Dex可呈剂量及时间依赖性下调ALI小鼠肺组织中的TREM-1mRNA的表达,并减少肺内髓样细胞胞膜TREM-1的脱落,提示Dex可能通过调节TREM-1的表达,从而抑制ALI早期的炎症级联反应,参与保护肺组织。
Objective To investigate the effect of Dexamethasone (Dex) on triggering receptor expressed on myeloid (IL-1) in lung tissue of mice with acute lung injury (ALI) induced by lipopolysaccharide (LPS) cells-1, TREM-1). Methods Kunming mice were injected with LPS (10 mg / kg) intraperitoneally to establish ALI model. Dex (5, 10, 20 and 40 mg / kg) mg / kg) for different time (6, 12, 24, 36 h). The expression of TREM-1 mRNA in lung tissue was detected by RT-PCR. The level of soluble TREM-1 protein in bronchoalveolar lavage fluid was detected by ELISA. Results Dex could reduce the pathological damage of lung. Dex could down-regulate the expression of TREM-1 mRNA in the lung of ALI mice in a time-dependent manner, and decreased at 6 h. Dex could down-regulate the expression of TREM- 1 mRNA expression and began to decrease at 10 mg / kg. Dex reduces the protein level of sTREM-1 in bronchoalveolar lavage fluid of ALI mice. Conclusion Dex can down-regulate the expression of TREM-1mRNA in the lung tissue of ALI mice in a dose-and time-dependent manner and reduce the shedding of TREM-1 in the lung myeloid cell membrane, suggesting that Dex may regulate the expression of TREM-1 Inhibit ALI early inflammatory cascade, involved in the protection of lung tissue.