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为研究不同来源人肿瘤细胞系表面cD137L的表达和功能。采用RT-PCR、FACS方法检测人CD137L在肿瘤细胞的表达,运用ELISA的方法检测CD137L对T细胞释放IFN-γ的影响及对肿瘤细胞分泌IL-8的影响。结果:来源于肝癌、肺癌、结肠癌、白血病的9种人肿瘤细胞系均可不同程度表达CD137L,但CD137L的表达水平与肿瘤细胞系的来源无相关,同一组织来源的细胞系,有的高表达,有的低表达。胚胎细胞系ECV 304中度表达CD137L,而正常新分离的PBMC不表达。功能研究发现:L78肿瘤细胞上表达的CD137L在CD3单抗存在的情况下与T细胞上的CD137L结合,能提高T细胞释放IFN-γ的水平,其作用具有剂量依赖性,这种作用可被抗-CD137L阻断;而CD137L表达水平与L78相似的Hep G2.2.15细胞在CD3单抗存在下并不能增强T细胞IFN-γ的分泌,进一步分析发现Hep G2.2.15同时表达较高水平的CD137(大约为91.6％),而L78几乎不表达CD137。肿瘤细胞上CD137L与表达在CHO细胞表面的CD137相互作用对肿瘤细胞分泌IL-8的水平产生不同的影响,使Hep G2.2.15分泌IL-8的水平约增加2倍(从15 pg/ml增加到30 pg/ml),但对肿瘤细胞HLE、A2分泌IL-8没有明显影响。CD137L在肿瘤细胞上的表达是普遍现象,其表达可能与细胞的快速生长有关;某些肿瘤细胞系表达的CD137L是有功能的,一方
To investigate the expression and function of cD137L on the surface of tumor cell lines from different origins. The expression of human CD137L in tumor cells was detected by RT-PCR and FACS. The effect of CD137L on the release of IFN-γ by T cells and the secretion of IL-8 by tumor cells were detected by ELISA. Results: Nine human tumor cell lines derived from liver cancer, lung cancer, colon cancer and leukemia all expressed CD137L to different extents, but the expression level of CD137L was not related to the origin of the tumor cell lines. In the same tissue-derived cell line, Expression, some low expression. CD137L was moderately expressed in the embryonic cell line ECV 304, whereas normal, newly isolated PBMCs were not expressed. Functional studies showed that CD137L expressed on L78 tumor cells binds to CD137L on T cells in the presence of CD3 mAb and increases the level of IFN-γ released by T cells in a dose-dependent manner and this effect can be Anti-CD137L. However, Hep G2.2.15 cells with CD137L expression level similar to L78 did not enhance the secretion of IFN-γ in T cells in the presence of CD3 mAb. Further analysis showed that Hep G2.2.15 expressed higher levels of CD137 (About 91.6%), whereas L78 hardly expressed CD137. CD137L on tumor cells and CD137 expressed on the surface of CHO cells have different effects on the secretion of IL-8 by tumor cells and about a 2-fold increase in the level of IL-8 secreted by Hep G2.2.15 (from 15 pg / ml To 30 pg / ml), but had no significant effect on the secretion of IL-8 by tumor cells HLE, A2. The expression of CD137L on tumor cells is a common phenomenon, and its expression may be related to the rapid growth of cells; CD137L expressed by some tumor cell lines is functional, and one