论文部分内容阅读
本研究建立的“蚕豆叶片法”、“黄瓜子叶法”和“萝卜块根法”是简便、快捷的杀菌剂生物测定法 .它们分别适用于水稻纹枯病 ( Pellicularia sasakii)、油菜菌核茎腐病 ( Sclerotinia sclerotiorum)和细菌性白菜软腐病 ( Erwinia ca-rotovora) .试材均以植物组织为特征 ,因此 ,也可称之为组织筛选法 .这些方法试材制备简单 ,操作技术容易掌握 ,温度和湿度等环境条件可局部控制 ,重复性强 ,在短时间内可对大量样品进行活性检测 .通过对多种化合物测试 ,表明这些方法与常规植株筛选法具有很高的相关性 .本文建立的“对峙培养法”是一种针对天然物抗菌活性检测法 ,可适用于对微量分离物活性跟踪检测
The “Broad bean leaf method”, “Cucumber cotyledon method” and “Radish root tuber method” established in this study are convenient and rapid bioassay methods for fungicides.They are suitable for Pellicularia sasakii, Sclerotinia sclerotiorum and Erwinia ca- rotovora.The test materials are all characterized by plant tissues and therefore can also be referred to as tissue screening methods.The preparation of these test samples is simple and operation techniques are easy to grasp , Environmental conditions such as temperature and humidity can be partially controlled, and repeatability is strong, and a large number of samples can be tested for activity in a short period of time.Through testing a variety of compounds, these methods have a high correlation with routine plant screening methods. The establishment of the “confrontation culture Law” is a kind of antimicrobial activity against natural detection method can be applied to trace trace the activity of trace