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以1对同核异质的玉米C103为试材,进行了利用低浓度玉米小斑病菌T小种培养滤液作为激发子来提高玉米抗病性的研究。结果表明,经预处理叶片的病斑面积为(0.3±0.05)～(0.9±0.5)mm2,而对照为(23.1±8.7)mm2,差异极显著。同时还测定了与提高抗病性有关酶的变化,经0～96h的动态检测,以1∶50的处理效果为最好,苯丙氨酸解氨酶(PAL)活性比对照平均提高了64.2%,过氧化物酶(POD)活性比对照平均提高了41.2%,有害物质丙二醛(MDA)的含量比对照平均降低了29.7%。从而说明低浓度培养滤液本身能够成功地作为激发子来诱导玉米的系统获得性抗性。
A pair of isogenic corn C103 was used as experimental material to study the disease resistance of maize using the low concentration of the filtrate of T. minor. The results showed that the lesion area of the pretreated leaves was (0.3 ± 0.05) ~ (0.9 ± 0.5) mm2, while the control was (23.1 ± 8.7) mm2, the difference was significant. At the same time, the changes of enzymes related to disease resistance were also measured. The results showed that the activity of PAL at 1:50 was the best, and the activity of PAL was increased by 64.2 %, The activity of peroxidase (POD) increased by 41.2% on average and the content of malondialdehyde (MDA) decreased by 29.7% on average compared with the control. Thus indicating that the low concentration culture filtrate itself can successfully serve as an elicitor to induce systemic acquired resistance in maize.