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１．以纯化的ＹａｄＡ蛋白〔１、３〕免疫普通小鼠４只，获得小鼠ＹａｄＡ多克隆抗血清；２．利用上述免疫血清建立了ＹａｄＡ多克隆抗体的ＥＬＩＳＡ检测技术，并对其最适条件进行了观察；３．用纯化的ＹａｄＡ蛋白免疫ＢＡＬＢ／Ｃ小鼠２只，以免疫ＢＡＬＢ／Ｃ小鼠的脾细胞与骨髓瘤细胞系ＳＰ２／０融合，获得１７株分泌ＹａｄＡ－ＭｃＡｂ杂交瘤细胞株，将其中４株进行了再克隆，经过半年左右的反复传代及冷冻、复苏测试，抗体分泌稳定；４．Ｗｅｓｔｅｒｎ－ｂｌｏｔｉｎｇ分析表明瘤株所分泌的单克隆抗体特异；５．本研究研制的ＹａｄＡ单克隆抗体（ＹａｄＡ－ＭｃＡｂ）可能在布鲁氏菌与肠炎耶氏菌Ｏ∶９的鉴别诊断上有良好的应用前景
1. Four mice were immunized with purified YadA protein [1,3] to obtain mouse YadA polyclonal antiserum; 2. The ELISA assay of YadA polyclonal antibody was established by using the above immune serum and the optimal conditions were observed.3. Two BALB / C mice were immunized with the purified YadA protein, and the spleen cells of immunized BALB / c mice were fused with myeloma cell line SP2 / 0 to obtain 17 strains secreting YadA-McAb hybrid cells, of which 4 After re-cloning, after about six months of repeated passage and freezing, resuscitation test, antibody secretion stability; 4. Western-bloting analysis showed that the monoclonal antibody secreted by the tumor-specific; 5. The YadA monoclonal antibody (YadA-McAb) developed in this study may have a good application prospect in the differential diagnosis of Brucella and Yersinia enterocolitis O: 9