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以草莓为试材,采用接种丛枝菌根(AM)真菌和添加对羟基苯甲酸(PHBA)的方法,研究了根内球囊霉和摩西球囊霉对草莓自毒物质对羟基苯甲酸胁迫的缓解作用,即对草莓光合作用、根系结构和抗氧化物酶活性的影响。结果表明:0.5mmol·L~(-1) PHBA浓度时,接种根内球囊霉显著提高了草莓净光合速率、气孔导度、胞间CO_2浓度和蒸腾速率;摩西球囊霉显著提高了草莓的净光合速率、胞间CO_2浓度和瞬时水分利用效率;根内球囊霉明显提高了草莓实际光化学效率(Ф_(PSII))、荧光淬灭系数(qP)、电子传递速率(ETR);摩西球囊霉提高了Ф_(PSII)和ETR。0.5mmol·L~(-1) PHBA浓度时,根内球囊霉明显增加了草莓根长、根投影面积、根表面积、根体积、根尖数、根分叉数,摩西球囊霉显著增加了根长和根尖数。0.5mmol·L~(-1) PHBA胁迫下,2株AM真菌均明显提高了草莓根部超氧化物歧化酶、过氧化物酶、过氧化氢酶活性。2株AM真菌通过增强草莓光合能力、根系发育和提高抗氧化物酶活性缓解了PHBA对草莓的毒害作用,根内球囊霉改善根系结构和提高抗氧化物酶活性的效果较摩西球囊霉明显,可能与菌根侵染率相关。
Strawberry was used as test material to study the effect of Glomus intracellularis and Glomus molitor on the self-toxicity of p-hydroxybenzoic acid to strawberries by inoculation of arbuscular mycorrhizal (AM) fungi and the addition of p-hydroxybenzoic acid (PHBA) Of the mitigation effect on the strawberry photosynthesis, root structure and antioxidant enzyme activity. The results showed that inoculation with Glomus intracellularis significantly increased net photosynthetic rate, stomatal conductance, intracellular CO 2 concentration and transpiration rate of 0.5 mmol·L -1 PHBA; (PII), fluorescence quenching coefficient (qP) and electron transfer rate (ETR) of strawberry were significantly increased. The photosynthetic rate of net photosynthetic rate, intercellular CO_2 and instantaneous water use efficiency Glomus increased Ф_ (PSII) and ETR. When 0.5 mmol·L -1 PHBA, the root growth rate of P. grisea significantly increased root length, root projection area, root surface area, root volume, root tip number, root bifurcation, The root length and root tips. Under the stress of 0.5 mmol·L -1 PHBA, the two AM fungi increased the activities of superoxide dismutase, peroxidase and catalase of strawberry roots significantly. The two AM fungi alleviates the toxic effects of PHBA on strawberry by enhancing their photosynthetic ability, root development and antioxidant enzyme activity. The results showed that the AM fungi could improve the root structure and enhance the antioxidant enzyme activity of G. globosum, Obviously, may be related to mycorrhizal infection rate.