实验性大鼠隐睾中eNOS的表达与生精细胞凋亡

来源 :华北煤炭医学院学报 | 被引量 : 0次 | 上传用户:wanglijun123456789
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(1)目的研究内皮型一氧化氮合酶(endothelialnitricoxidesynthase,eNOS)的表达与实验性大鼠隐睾生精细胞凋亡的关系。②方法通过手术建立单侧大鼠隐睾模型,术后第7天采用TUNEL法检测生精细胞凋亡,免疫组化SABC法及RT—PCR检测隐睾及对侧睾丸eNOS基因表达的变化。③结果术后第7天隐睾侧重量明显减轻,生精细胞凋亡指数较对侧睾丸明显增加;RT—PCR显示隐睾中eNOSmRNA的表述较对侧睾丸增强;免疫组化显示对侧睾丸生精上皮内未见eNOS表达,隐睾侧睾丸生精上皮中有eNO
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