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本组实验是以猫的小肠作低温灌注,在保养的48小时期间,间隔不同的时间,作形态和代谢方面检查,以观察低温灌注对肠产生何种病损以及与肠病损有关的代谢参数。方法:成年猫,体重为2.2～4.1kg,取保留肠系膜上动、静脉在内的一段50cm小肠,离体后供灌注。先用4℃林格氏醋酸溶液清洗肠腔后,结扎其近侧端。在肠系膜上动脉内插入聚乙烯导管,静脉端则任其开放。用含肝素1万单位/l和普鲁卡因1g/l的4℃林格氏醋酸溶液,鼓氧后冲洗血管,直至从静脉的流出液澄清。将
In this experiment, the cat’s small intestine was hypothermia perfusion. During the 48 hours of maintenance, different intervals of time, morphological and metabolic examinations were performed to observe the effects of hypothermic perfusion on intestinal damage and metabolism associated with intestinal lesions parameter. Methods: Adult cats, weighing 2.2 ~ 4.1kg, retained the upper mesenteric artery and veins, including a section of 50cm small intestine, isolated for perfusion. First with 4 ℃ Ringer ’s acetic acid solution after cleaning the intestinal lumen, ligation of its proximal end. Polyethylene catheters were inserted into the superior mesenteric artery, while the venous end was left open. Blood was rinsed with oxygen in a 4 ° C Ringer & apos; s solution containing heparin 10,000 / l and procaine 1 g / l until the effluent from the vein was clarified. will