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用免疫细胞化学ABC法和镀银复染,研究人胎(孕龄为16～28周的人流标本)小脑酪氨酸羟化酶(tyro-sine hydrolyse,TH)和多巴胺β-羟化酶(dopamine β-hydrolyse,DβH)神经元及其传入纤维的发育.结果证实,16～18周龄,两种免疫性反应已出现于小脑皮质的分子层,Purkinje细胞和粒细胞层.23周,TH和DβH阳性Purkinje细胞排列紧密,其核周质含粗大颗粒状的呈色物明显地延伸至突起内,此外,分子层尚见有棱形的蓝细胞和圆形的星形细胞均有散在的阳性反应.26～28周,皮质各阳性胞体的突起明显增长,胞体增大.切片镀银复染,16周龄的皮质亦已见有丰富的苔状纤维和少量的平行纤维存在;随胎龄发育,TH和DβH两种阳性Purkinje细胞胞体和突起均还有较丰富的爬行纤维附着;且分子层内可见更多的平行纤维与阳性的Purkinje细胞的突起相交错成网.小脑中央各核,16～18周龄已明显有TH和DβH的阳性反应,且随胎龄的发育,各核群(顶核、球核、栓核和齿状核)的阳性胞体各异,而其各胞体的直径大小,则始终是以齿状核的胞体为最小.切片镀银复染,在本文的最早胎龄(16周)亦已见大量的传入纤维布于中央各核,在阳性胞体上均附有传入纤维的突触样小结;23周龄后,中央核内阳性胞体与小脑皮质、前庭脊核,以及参与小脑脚的纤维联系,所见更为丰富和显著
Immunocytochemical ABC method and silver plating counterstaining were used to study the effect of tyrosine hydroxylase (TH) and dopamine β-hydroxylase (HBeAg) on human fetus (gestational age from 16 to 28 weeks) dopamine β-hydrolyse, DβH) neurons and their afferent fibers. The results showed that at 16-18 weeks of age, two immune responses have emerged in the cerebellar cortex, Purkinje and granulocyte layers. At 23 weeks, TH and DβH-positive Purkinje cells arranged closely, the perinuclear macrophages containing coarse particles significantly extended into the protuberance, in addition, the molecular layer still see the prismatic blue cells and round astrocytes are scattered Of the positive reaction .26 to 28 weeks, the cortical positive cell bodies significantly increased the process of protrusion, cell body increased section of silver staining, 16-week-old cortex has also seen a rich moss-like fibers and a small amount of parallel fibers; The gestational age, TH and DβH two positive Purkinje cell body and process are still rich in crawling fiber attachment; and the molecular layer can be seen more parallel fibers and the positive Purkinje cell processes staggered into the network. Nuclear, 16 to 18 weeks of age have been significantly positive TH and DβH, and with the fetus The development of age, the nuclei of various nuclei (the top nuclear, nuclear, thrombus and dentate nuclei) positive cell bodies vary, and the size of each cell diameter, then the germ cell is always the smallest of the body. In the first part of this study, a large number of afferent fibers were also found in the central nuclei. Synaptic nodules with afferent fibers were attached to the positive soma. After 23 weeks of age, the central nucleus Internal positive soma with cerebellar cortex, vestibular nucleus, and involved in cerebellar pedicel fibers, the richer and more significant