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目的通过观察血清超敏C反应蛋白(high-sensitivity C-reactive protein,hsCRP)水平的变化探讨牙周基础治疗对伴或不伴高脂血症的慢性牙周炎(chronic periodontitis,CP)大鼠动脉粥样硬化(atherosclerosis,As)发生的影响。方法本研究于2011年5—9月在山西医科大学口腔医学院完成。将24只6周龄雄性SD大鼠随机分为4组,即正常对照组(A组)、高脂血症组(B组)、CP组(C组)和高脂血症+CP组(D组),每组6只。各组接受相应的建模及牙周干预处理,同时定期进行牙周临床检查,酶联免疫吸附(ELISA)法检测牙周干预前及每次干预后1周(共2次)3个取样时间点的血清hsCRP水平。结果牙周临床检查结果显示,整个实验过程中A、B组大鼠实验牙牙龈未见炎症性改变;C、D组大鼠建模后实验牙牙龈炎症明显,有深牙周袋,部分实验牙松动度达Ⅱ~Ⅲ度,基础治疗后炎症明显减轻,牙齿均无松动。同一时间点上,B、C及D组大鼠血清hsCRP水平均显著高于A组,差异有统计学意义(P<0.01)。在不同时间点上,B、C及D组大鼠治疗后血清hsCRP水平均较治疗前显著增高,差异有统计学意义(P<0.01)。其中,C组大鼠2次治疗后血清hsCRP水平趋于稳定,D组大鼠在2次治疗后1周血清hsCRP水平显著增高。结论对于慢性牙周炎大鼠,无高脂血症情况存在时,牙周基础治疗可能会在短期内增高As的发生风险;在存在高脂血症状态下,直接牙周基础治疗可能会在较长时间内增高As的发生风险。
Objective To observe the changes of serum hsCRP levels in patients with chronic periodontitis (CP) treated with or without hyperlipidemia The effect of atherosclerosis (As). Methods This study was performed in the School of Stomatology, Shanxi Medical University from May to September, 2011. Twenty-four male Sprague-Dawley rats of 6 weeks old were randomly divided into 4 groups: normal control group (group A), hyperlipidemia group (group B), CP group (group C) and hyperlipidemia + CP group D group), 6 in each group. The rats in each group were subjected to the corresponding modeling and periodontal intervention. Periodontal clinical examination was performed at the same time. Before the periodontal intervention and 1 week after each intervention (2 times in total), three sampling times were detected by enzyme linked immunosorbent assay (ELISA) Point serum hsCRP levels. Results The results of periodontal examination showed that there was no inflammatory change in the gingival of test teeth in groups A and B during the whole experiment. The gingival inflammation of experimental teeth was obvious after modeling in groups C and D, with deep periodontal pockets and some experiments Tooth mobility of Ⅱ ~ Ⅲ degree, the basic treatment significantly reduced inflammation, no loose teeth. At the same time point, serum hsCRP levels in rats in groups B, C and D were significantly higher than those in group A (P <0.01). At different time points, the serum hsCRP levels of rats in groups B, C and D after treatment were significantly higher than those before treatment, with statistical significance (P <0.01). Among them, serum hsCRP levels of rats in group C after two treatments tended to be stable, and serum hsCRP levels of group D were significantly increased at one week after two treatments. Conclusion In the absence of hyperlipidemia in rats with chronic periodontitis, periodontal treatment may increase the risk of As in the short term. In the presence of hyperlipidemia, direct periodontal treatment may be at For a long time increase the risk of As.