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目的 探讨在MALT淋巴瘤石蜡组织中检测API2 MALT1融合基因的可行性及API2 MALT1融合基因mRNA表达在肺MALT淋巴瘤诊断中的意义。方法 收集肺MALT淋巴瘤石蜡包埋组织标本 10例 ,以慢性淋巴结炎石蜡包埋组织标本 5例作阴性对照 ,β 肌动蛋白作内对照 ,用逆转录 聚合酶链反应 (RT PCR)和巢式PCR结合 ,检测肺MALT淋巴瘤中API2 MALT1融合基因的mRNA表达。结果 10例肺MALT淋巴瘤均检出内对照β actin的mRNA表达 ,3例检出API2 MALT1融合基因的mRNA表达 ,被检出的 3例病变均局限在一侧肺组织 ,单个病灶最大径小于 5cm。其中 2例出现不同程度的呼吸道症状 ,1例为体检发现 ,所有慢性淋巴结炎病例均未检出融合基因的表达。结论通过RT PCR和PCR扩增结合的方法检测石蜡包埋组织中API2 MALT1融合基因的表达是完全可行的 ,API2 MALT1融合基因的表达与MALT淋巴瘤的病变范围有一定关系
Objective To investigate the feasibility of detecting API2 MALT1 fusion gene in MALT lymphoma paraffin tissues and the significance of API2 MALT1 fusion gene mRNA expression in the diagnosis of lung MALT lymphoma. Methods 10 paraffin-embedded tissue specimens of lung MALT lymphoma were collected. Five paraffin-embedded specimens of chronic lymphocytic disease were used as negative control and β-actin as control. Reverse transcription-polymerase chain reaction (RT PCR) PCR was performed to detect the mRNA expression of API2 MALT1 fusion gene in lung MALT lymphoma. Results The mRNA expression of β actin was detected in 10 cases of lung MALT lymphoma. The mRNA expression of the API2 MALT1 fusion gene was detected in 3 cases. All the 3 lesions were detected in one side of lung tissue. The maximum diameter of single lesion was less than 5cm. Two cases showed different degrees of respiratory symptoms, one case found for physical examination, all cases of chronic lymphadenitis did not detect fusion gene expression. Conclusion It is feasible to detect the expression of API2 MALT1 fusion gene in paraffin-embedded tissues by RT-PCR and PCR amplification. The expression of API2 MALT1 fusion gene has a certain relationship with the extent of MALT lymphoma