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[目的]探讨前列腺素E1(PGE1)对急性心肌梗死(AMI)大鼠心肌血管内皮生长因子(VEGF)表达的影响。[方法]健康雄性SD大鼠50只分为PGE1处理组(n=40)、生理盐水对照组(n=10)和假手术组(n=10),PGE1处理组分为4个亚组,每组10只,分别采用不同剂量PGE1每日静脉注射,手术后2周和4周处死大鼠,取心脏标本切片,HE染色及VEGF免疫组化染色后显微镜观察,分析心肌VEGF蛋白表达水平。[结果]PGE1处理组VEGF蛋白的表达显著增加,明显高于对照组和假手术组,差异有统计学意义(P﹤0.05)。随着PGE1剂量的增加,VEGF蛋白表达水平逐渐升高。[结论]PGE1可以增加AMI大鼠心肌VEGF蛋白表达,促进侧支循环建立。PGE1引起的心肌VEGF蛋白表达升高作用呈剂量依赖性。
[Objective] To investigate the effect of prostaglandin E1 (PGE1) on the expression of vascular endothelial growth factor (VEGF) in rats with acute myocardial infarction (AMI). [Methods] Fifty healthy male Sprague-Dawley rats were divided into 4 groups: PGE1 treatment group (n = 40), saline control group (n = 10) and sham operation group (n = 10) The rats in each group were given daily intravenous injection of PGE1 at different doses. The rats were sacrificed at 2 weeks and 4 weeks after operation. The heart specimens were obtained and stained with HE staining and VEGF immunohistochemistry. The expression of VEGF protein in myocardium was analyzed. [Results] The expression of VEGF protein in PGE1 treatment group was significantly increased, which was significantly higher than that in control group and sham operation group (P <0.05). With the increase of PGE1 dose, the expression of VEGF protein gradually increased. [Conclusion] PGE1 can increase the expression of VEGF protein in myocardium of AMI rats and promote the establishment of collateral circulation. PGE1-induced myocardial VEGF protein expression increased in a dose-dependent manner.