锌原卟啉作为环境铅污染人群筛查指标的可行性研究

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目的探讨红细胞锌原卟啉(ZPP)应用于人群接触环境铅污染筛查指标的可行性。方法以紫金县某电池厂附近常住居民为调查对象,采集被调查者静脉血2~3 mL,采用血液锌原卟啉测定仪测定ZPP、石墨炉原子吸收光谱法测定血铅。儿童以血铅含量≥100μg/L、成年人以≥400μg/L为慢性铅中毒判定标准。结果共调查946名居民,其中1~13岁儿童174人、16~87岁成年人772人。儿童血铅含量超标率为14.9%(26/174),儿童ZPP含量中位数为0.780μmol/L,血铅中位数为47.675μg/L,儿童ZPP含量与血铅含量的相关关系没有统计学意义(P>0.05)。成人血铅含量超标率为9.5%(73/772),成人ZPP含量中位数为0.740μmol/L,血铅中位数为69.572μg/L,成人ZPP含量与血铅含量呈弱相关关系(r=0.344,P<0.05)。儿童血铅高水平组(血铅≥100μg/L)ZPP含量与血铅含量呈较强的相关关系(r=0.530,P<0.05),儿童血铅低水平组(血铅<100μg/L)ZPP含量与血铅含量的相关关系没有统计学意义(P>0.05)。成人血铅高水平组(血铅≥400μg/L)和低水平组(血铅<400μg/L)的ZPP含量与血铅含量均具有相关关系(r分别为0.566、0.142,均P<0.05)。结论成人血铅或儿童血铅水平较高时,ZPP可以作为环境铅污染人群筛查的指标。 Objective To investigate the feasibility of using zinc oxide porphyrin (ZPP) as a marker of lead pollution in human contact environment. Methods The residents living nearby a battery factory in Zijin County were investigated. Blood samples were collected from 2 to 3 mL of venous blood and ZPP was measured using a zinc protoporphyrin analyzer. Blood lead levels were determined by graphite furnace atomic absorption spectrometry. Children with blood lead levels ≥ 100μg / L, adults with ≥ 400μg / L as a chronic lead poisoning criteria. Results A total of 946 residents were surveyed, including 174 children aged from 1 to 13 and 772 adults aged from 16 to 87. The blood lead level in children exceeded the rate of 14.9% (26/174), the median of children with ZPP was 0.780μmol / L, the median of blood lead was 47.675μg / L, the correlation between children’s ZPP content and blood lead content was not statistically Significance (P> 0.05). The blood lead level in adults exceeded the rate of 9.5% (73/772), the median adult ZPP concentration was 0.740μmol / L, the median blood lead was 69.572μg / L, adult ZPP content and blood lead content was weakly related r = 0.344, P <0.05). Children with high level of blood lead (blood lead ≥100μg / L) ZBP content and blood lead was strongly correlated (r = 0.530, P <0.05), children with low blood lead levels (blood lead <100μg / L) The correlation between ZPP level and blood lead level was not statistically significant (P> 0.05). ZPP content in blood lead level (blood lead≥400μg / L) and low level group (blood lead <400μg / L) were correlated with blood lead levels (r = 0.566,0.142, P <0.05, respectively) . Conclusion ZPP can be used as an indicator of environmental lead contamination in adults when blood lead or blood lead levels in children are high.
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