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目的对2010-2013年杭州地区甲型流感H3N2进行分子流行病学调查分析。方法收集2010-2013年杭州地区H3N2监测样本数据,对样本数据按年份、性别以及年龄进行分组,比较各组之间的差异。查询、整理GenBank中的中国H3N2序列并建立数据集,并对国内H3N2的HA基因及2010-2013年杭州地区H3N2病毒HA、NA序列进行系统生物进化分析。结果 2010-2013年杭州地区H3N2感染率分析,不同年度间H3N2感染率差异具有统计学意义(χ2=14.004,P<0.05),不同性别和年龄组间差异无统计学意义(χ2=3.552,χ2=2.691,P>0.05)。杭州地区2010年与2012、2013年H3N2感染病毒株,在系统进化树上分布存在两个不同分支,且HA序列进化属于广东省H3N2进化的不同时间点的两个分支,在国内H3N2系统进化树上存在一类未见人类感染的病毒分支。结论 H3N2感染无性别选择性及年龄差异,不同年度的感染率存在差异;杭州及我国多省市流行的H3N2感染存在广泛迁徙的现象,今后仍然具有引起高致病性大规模流行的能力,因此应加强各地监测及信息共享,做到早发现和及时防控。
Objective To investigate the molecular epidemiology of influenza A (H3N2) in Hangzhou during 2010-2013. Methods The data of H3N2 surveillance samples from 2010 to 2013 in Hangzhou area were collected. The sample data were grouped by age, gender and age, and the differences among the groups were compared. The Chinese H3N2 sequence in GenBank was screened and the dataset was established. The domesticated H3N2 HA gene and H3N2 HA and NA sequences from 2010 to 2013 in Hangzhou were systematically analyzed. Results The prevalence of H3N2 infection in Hangzhou during 2010-2013 was significantly lower than that in other years (χ2 = 14.004, P <0.05). There was no significant difference between genders and age groups (χ2 = 3.552, χ2 = 2.691, P> 0.05). There were two different branches in the phylogenetic tree of H3N2 infected strains in Hangzhou in 2010, 2012 and 2013, and the evolution of HA sequences belonged to two branches at different time points of evolution of H3N2 in Guangdong Province. In the domestic H3N2 phylogenetic tree There is a class of virus that is free from human infection. Conclusions H3N2 infection has no gender-selective and age-related differences in infection rates in different years. H3N2 prevalence in Hangzhou and other provinces in China is widespread and still possesses the ability to cause HPAI pandemics in the future. Therefore, Monitoring and information sharing should be strengthened everywhere to achieve early detection and timely prevention and control.