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为鉴定柞蚕蛹虫草新的医用价值,将不同浓度的柞蚕蛹虫草水提物(AEoAPC)分别作用于体外培养的人肝癌SMMC-7721细胞,于24、48、72 h后采用倒置相差显微镜和透射电子显微镜观察、四甲基偶唑蓝(MTT)比色及流式细胞术(FCM)检测细胞的增殖和凋亡情况。结果表明:细胞培养板中分别加入质量浓度为0.1、0.2、0.5、0.8、1.0、2.0、5.0 g/L的AEoAPC,均能显著抑制SMMC-7721细胞的增殖,并呈现时间剂量依赖性,1.0 g/L AEoAPC处理24、48、72 h后,对SMMC-7721细胞的生长抑制率分别为18.9%、46.4%、77.2%;经AEoAPC处理后大量癌细胞出现染色质边集和凝聚、核碎裂及凋亡小体;1.0 g/LAEoAPC处理24、48、72 h SMMC-7721细胞凋亡率分别为7.65%、11.04%、23.02%,细胞凋亡程度与AEoAPC作用时间呈正相关,有丝分裂第一间距期(G1)的细胞在AEoAPC作用72 h后分布明显增加。研究结果初步显示,AEoAPC对人肝癌SMMC-7721细胞有抑制增殖和诱导凋亡的作用,其抗肿瘤机制与诱导肿瘤细胞凋亡有关。
In order to identify the new medical value of the silkworm Cordyceps militaris, different concentrations of water extract of tussah Cordyceps militaris (AEoAPC) were applied to human hepatocellular carcinoma SMMC-7721 cells cultured in vitro, after 24,48,72 h inverted phase contrast microscopy and transmission Electron microscopy, MTT colorimetric and flow cytometry (FCM) were used to detect cell proliferation and apoptosis. The results showed that AEoAPC with 0.1,0.2,0.5,0.8,1.0,2.0,5.0 g / L, respectively, could significantly inhibit the proliferation of SMMC-7721 cells in a dose-dependent and time-dependent manner The growth inhibition rates of SMMC-7721 cells treated with AEoAPC for 24, 48 and 72 h were 18.9%, 46.4% and 77.2%, respectively. After treatment with AEoAPC, a large number of cancer cells showed chromatin margination and condensation The apoptotic rates of SMMC-7721 cells treated with 1.0 g / LAEoAPC for 24, 48 and 72 h were 7.65%, 11.04% and 23.02%, respectively. The degree of apoptosis was positively correlated with the duration of AEoAPC, The interval (G1) of the cells in the AEoAPC after 72 h distribution increased significantly. The results showed that AEoAPC could inhibit proliferation and induce apoptosis of human hepatocellular carcinoma SMMC-7721 cells. The anti-tumor mechanism of AEoAPC is related to inducing apoptosis of tumor cells.