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建立了反相高效液相色谱-二极管阵列检测法同时测定毛冬青根中的6种活性成分(tortoside A,(+)-丁香脂素,毛冬青皂苷B3,毛冬青皂苷A1,毛冬青皂苷B1,毛冬青素A)的含量.RP-HPLC分离用反相C18分析柱,流动相以乙腈-0.1％磷酸水体系梯度洗脱.流速1.0 mL/min,检测波长为210 nm.6个指标成分达到基线分离,标准曲线具有良好的线性(r2＞0.9996),精密度、稳定性和重复性符合分析方法学要求.加样回收率范围为99.00％-104.52％.最后对15个批次的毛冬青药材含量测定结果进行了聚类分析.结果表明该方法不仅可以对毛冬青药材进行质量控制,而且也为毛冬青药材的道地性分析提供了科学依据.“,”A reversed-phase high performance liquid chromatography (RP-HPLC) method with diode array detector (DAD) has been established to simultaneously determine six bioactive compounds in the roots of Ilex pubescens,namely tortoside A,(+)-syringaresinol,ilexsaponin B3,ilexsaponin A1,ilexsaponin B1,ilexgenin A.The RP-HPLC assay was performed on a reversedphase C18 column with a gradient elution.The mobile phase consisted of acetonitrile and water containing 0.1％ (v/v) phosphoric acid.The flow rate was 1.0 mL/min and the detection wavelength was set at 210 nm.The six marker constituents were separated well with good linearity (r2＞0.9996),precision,stability and repeatability.The overall recoveries were in the range of 99.00％-104.52％.Cluster analysis was employed to analyze 15 batches of samples.The result indicated this method provide an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication ofⅠ.pubescens.