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以桃‘红垂枝’与其近缘种山桃‘白花山碧桃’杂交的52株F1群体为试材,采用SSR、AFLP和SRAP分子标记进行遗传分析。筛选扩增稳定且多态性丰富的38对SSR引物、61对AFLP引物和38对SRAP引物进行群体分离分析,获得符合1:1分离的标记441个,亲本为双杂合位点的标记52个。应用Mapmaker分析软件将符合1:1分离的标记和雌蕊发育性状一起构建了包含11个连锁群的遗传图谱,该图谱共206个标记(18个SSR,126个AFLP、61个SRAP和1个形态学标记),全长1193.2cM。每个连锁群的平均长度为108.5cM,标记间平均图距为5.8cM。根据18个SSR标记与T×E参考图谱比对,本试验中的11个连锁群对应于参考图谱的G1至G8,标记的线性符合度较好。雌蕊发育性状标记定位在第1连锁群,对应于1号染色体。应用Mapmaker分析软件将亲本为双杂合位点标记和花单瓣/重瓣性状一起分析,获得1个新单瓣/重瓣基因的两个AFLP标记。
Fifty - two F1 hybrids were crossed with the peach ’Red weeping sticks’ and its neighboring species, ’Peach Blossom Mountain’, and SSR, AFLP and SRAP markers were used for genetic analysis. Thirty-eight pairs of SSR primers with stable amplification and rich polymorphism were screened, 61 pairs of AFLP primers and 38 pairs of SRAP primers were screened for population segregation analysis, and 441 markers with 1: 1 segregation and 52 markers with two-hybrid parents A Using Mapmaker analysis software, a 1: 1 segregated marker and pistil development trait were used to construct a genetic map containing 11 linkage groups with 206 markers (18 SSRs, 126 AFLPs, 61 SRAPs and 1 morphological pattern Learn mark), full-length 1193.2cM. The average length of each linkage group was 108.5 cM and the average distance between markers was 5.8 cM. According to the comparison of 18 SSR markers with the T × E reference map, 11 linkage groups in this experiment correspond to G1 to G8 in the reference map, and the linearity of the markers is good. Pistil development trait markers located in the first linkage group, corresponding to chromosome 1. Mapmaker analysis software was used to analyze the parents with two-hybrid loci markers and flower single / double trait traits to obtain two AFLP markers of a new single / double gene.