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目的胶质瘤的化疗一直都是研究热点,近来有研究表明甲基化与胶质瘤的发生和发展关系密切,本研究的目的是探讨去甲基化试剂5-氮杂-2’-脱氧胞苷(5-Aza-2’-deoxycytidine)和曲古霉素A(TSA)联合应用对胶质瘤细胞系侵袭和转移的调节。方法分别将浓度为50μM的5-氮杂-2’-脱氧胞苷和300ng/ml的曲古霉素A加入胶质瘤细胞系U251细胞中,并将两者联合应用加入U251细胞系中,应用MTT法检测不同处理组细胞的增殖能力,Transwell细胞侵袭实验检测不同处理组和对照组中细胞的侵袭能力的变化。结果 5-氮杂-2’-脱氧胞苷和曲古霉素A单独处理后,U251细胞的增殖能力明显减弱(<0.05),两者联合应用时对U251细胞的增殖抑制更明显(<0.01)。Transwell细胞侵袭实验显示5-氮杂-2’-脱氧胞苷和曲古霉素A单独处理后,U251细胞的侵袭细胞数明显减少(<0.05),而两者联合应用时对U251细胞的侵袭能力抑制更为明显(P<0.01)。结论 5-氮杂-2’-脱氧胞苷和曲古霉素A都可以抑制胶质瘤细胞系的增殖和侵袭,两者具有协同作用。
The purpose of glioma chemotherapy has been a research hot spot, recent studies have shown that methylation and glioma occurrence and development are closely related, the purpose of this study is to explore the demethylating agent 5-aza-2’-deoxy Regulation of Invasion and Metastasis of Glioma Cell Lines by Combining 5-Aza-2’-deoxycytidine and Trichostatin A (TSA). Methods 5-aza-2’-deoxycytidine and 300ng / ml of curcumin A were respectively added into the glioma cell line U251 at a concentration of 50μM, and the two were added into the U251 cell line. The proliferation of cells in different treatment groups was detected by MTT method. The invasion ability of cells in different treatment groups and control groups was detected by Transwell cell invasion assay. Results After treatment with 5-aza-2’-deoxycytidine and trichostatin A alone, the proliferation of U251 cells was significantly weakened (P <0.05), and the inhibition of U251 cells proliferation was more significant (<0.01 ). Transwell cell invasion assay showed that the number of invasive cells in U251 cells was significantly decreased (P <0.05) after treatment with 5-AZA-2’-deoxycytidine and monacolin A alone, but the combination of the two could affect the invasion of U251 cells Ability to inhibit more significantly (P <0.01). Conclusion Both 5-AZA-2’-deoxycytidine and trichostatin A can inhibit the proliferation and invasion of glioma cell lines, and they have synergistic effects.