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采用两种抗原 (抗原 B和粗抗原 )通过 EL ISA法检测感染细粒棘球蚴和多房棘球蚴 2～ 3月的小鼠、正常鼠以及棘球蚴病人、非棘球蚴病人和健康人血清抗体。结果表明 ,抗原 B和粗抗原检测 2月细粒棘球蚴病鼠血清抗体阳性率分别为 95 %和 10 0 % (P>0 .0 5 )。检测 3月细粒棘球蚴病鼠血清抗体均为 10 0 % ,检测正常鼠血清的假阳性率分别为 0和 5 %(P>0 .0 5 )。检测 3月泡球蚴病鼠的假阳性率分别为 38.46 %和 84.6 2 % (P<0 .0 1)。抗原 B和粗抗原检测 37例细粒棘球蚴病患者血清抗体的阳性率为 10 0 % ,而检测 6 1例非细粒棘球蚴病患者血清抗体的假阳性率为 6 .6 6 %和 19.6 7% (P<0 .0 5 ) ,检测健康人血清的假阳性率分别是 0和 5 % (P>0 .0 5 )。结果提示 ,抗原 B诊断早期棘球蚴病鼠的敏感性与粗抗原相似 ,但特异性优于囊液粗抗原。
Two kinds of antigens (antigen B and crude antigen) were detected by EL ISA method to infect Echinococcus granulosus and Echinococcus multilocularis 2-3 month mice, normal mice and hydatid disease patients, non-hydatid disease patients and Healthy human serum antibody. The results showed that the positive rates of serum antibody in the test of antigen of B and crude antigen in Feb. were 95% and 100% respectively (P> 0.05). Serum antibodies of 100% of mice with Echinococcus granulosus were detected in March, and the false-positive rates of serum in normal mice were 0% and 5%, respectively (P> 0.05). The false-positive rates of detecting 3-week-long alveolar echinococcosis mice were 38.46% and 84.62%, respectively (P <0.01). The positive rate of serum antibody in 37 cases of patients with Echinococcus granulosus was 10% detected by antigen B and crude antigen, while the false positive rate of serum antibodies in 61 cases with non-Echinococcosis was 6.66% And 19.6 7% (P <0.05) respectively. The false-positive rates of serum in healthy volunteers were 0 and 5%, respectively (P> 0.05). The results suggest that the antigen B antigen in early diagnosis of early hydatidosis and rough antigen similar sensitivity, but the specificity is better than the cyst fluid crude antigen.