,Endothelial cells modified by adenovirus vector containing nine copies hypoxia response elements an

• 作 者: Xiaobin Song Liang Shi Lamei Chen Xinyu Liu Xun Qu Ketao Wang Fengcai Wei
• 机 构: Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan 250012, Ch
• 出 处: 生物化学与生物物理学报（英文版）
• 发表日期: 2017年11期
• 关键词: vascular endothelial growth factor hypoxic response element bone tissue engineer

Vascularization is one of the hotspots during the development of new therapeutic strategies for bone tissue engineering,which can alleviate hypoxic circumstance and prevent transplant failure.Vascular endothelial growth factor (VEGF) gene transfection using recombinant adenovirus (Ad) vector can effectively promote angiogenesis,but uncontrolled long-term continuous expression of VEGF brings safety conce.Here we constructed a recombinant Ad vector containing nine copies of HRE promoter and the hVEGF165 gene,which conserved the oxygen sensitivity of hypoxiainducible factor-1/hypoxia response elements (HIF-1/HRE).After transfection into human umbilical vein endothelial cells (HUVEC),the hVEGF165 mRNA and protein levels were much higher in response to hypoxia,as revealed by RT-PCR and ELISA,respectively.Furthermore,Ad-9HREhVEGF165 vector effectively promoted proliferation,migration and tube formation of HUVEC under hypoxic conditions.Thus we believe that the Ad-9HRE-hVEGF165 vector can contribute to the regulation of vascularization,which may provide a new approach for a better control of the expression of hVEGF165 during bone tissue engineering.