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目的:分析外周血游离Epstein-Barr病毒(EBV)是否特异地出现于鼻咽癌患者。方法:收集鼻咽癌患者和非鼻咽癌肿瘤患者血浆各50例,正常人血浆30例,提取DNA,荧光定量PCR检测EBV拷贝数,比较游离EBV在上述人群的差异。结果:在25例(50%)鼻咽癌患者血浆中检测到游离EBV,其拷贝数波动于1×103拷贝/ml血浆～2.6×106拷贝/ml血浆,中位数为5.3×105拷贝/ml血浆。在50例非鼻咽癌肿瘤患者和30例正常人血浆中均未检测到游离EBV。结论:游离EBV出现于鼻咽癌患者外周血,可能是一个较特异的鼻咽癌标志物。
Objective: To analyze whether peripheral blood Epstein-Barr virus (EBV) is specifically expressed in nasopharyngeal carcinoma patients. Methods: The plasma samples from patients with nasopharyngeal carcinoma and non-nasopharyngeal carcinoma were collected from 50 patients with normal plasma and 30 healthy individuals. DNA was extracted and the copy number of EBV was detected by real-time quantitative PCR. The differences of free EBV in these populations were compared. RESULTS: Free EBV was detected in plasma from 25 patients (50%) of NPC patients with a copy number fluctuation of 1 × 10 3 copies / ml plasma to 2.6 × 10 6 copies / ml plasma with a median of 5.3 × 10 5 copies / ml plasma. Free EBV was not detected in plasma of 50 non-NPC patients and 30 normal controls. Conclusion: Free EBV appears in the peripheral blood of patients with nasopharyngeal carcinoma, which may be a more specific marker of nasopharyngeal carcinoma.