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用已知的拟南芥抗病性基因的8对引物对60个芸薹属作物和1份拟南芥品系进行了遗传多样性分析，除GPA1，PR1外，其他6对引物的同源片断都被检测到。其中与Atox1，BGL2，RPS2及PG11四个基因同源的片断检出率较高，分别为62．5％，94．7％，87．5％及100．0％。用这些同源片断估计了甘蓝型油菜抗耐菌核病材料中油821、中R783、中R888、宁RS－1之间的遗传距离。聚类分析结果表明，中R783与中R888的遗传距离较近，为0．0323。中R783，中R888与宁RS-13个抗病品系之间的遗传距离为0．1007。这3个品系与中油821的遗传距离为0．2463，与拟南芥的遗传距离为0．4979。由于籍以进行聚类分析的遗传标记是与抗病有关基因同源的DNA序列，因此，4个甘蓝型油菜抗病品系间的遗传距离应当或多或少反应了它们之间在抗病性遗传基础上的差别。从61份DNA样品中扩增出与BGL2和RPS2同源的多态性片断的数据合并作聚类分析，在这两个基因同源序列的多样性上，芸薹属作物与拟南芥相似性为44％，60份芸薹属作物供试材料在56％的水平上聚为一类。在相似性为70％的水平上可将这些材料分为5个组：I．包括1个黑齐品种和所有参与测试的6个甘蓝，1个埃芥品系和23个甘蓝型油菜品种。II．1个埃芥品系和14个甘蓝型油菜品种。III．有7个甘蓝型油?
Genetic diversity analysis was carried out on 60 Brassica crops and 1 Arabidopsis thaliana strain using 8 pairs of primers of known Arabidopsis thaliana resistance genes. The homologous fragments of 6 primer pairs except GPA1 and PR1 Have been detected. Among them, the homology of Atox1, BGL2, RPS2 and PG11 was higher than that of Atox1, BGL2, RPS2 and PG11, which were 62.5%, 94.7%, 87.5% and 100.0% respectively. Using these homologous fragments, the genetic distance between oilseeds 821, middle R783, middle R888, and Ning RS-1 in Brassica napus resistant to B. avium was estimated. Cluster analysis showed that the genetic distance between R783 and R888 was close to 0.0323. The genetic distance between R783, R888 and Ning RS-13 resistant strains was 0.1007. The genetic distance between these 3 lines and Zhongyou 821 was 0.2463, and the genetic distance to Arabidopsis was 0.4979. Since the genetic markers used for clustering analysis are DNA sequences homologous to the disease-resistant genes, the genetic distance between the four Brassica napus resistant lines should reflect more or less the disease resistance Genetic differences. Data from 61 DNA samples amplified polymorphic fragments homologous to BGL2 and RPS2 were pooled for clustering analysis and Brassica crops were similar to Arabidopsis on the diversity of homologous sequences of these two genes The sex was 44%, and 60 samples of Brassica crops were grouped at 56% level. These materials can be divided into five groups at a similarity level of 70%: I. Including a black breed and all involved in the test of 6 cabbage, 1 mustard lines and 23 varieties of Brassica napus. II. 1 mustard strain and 14 varieties of Brassica napus. III. There are seven kale oil?