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目的:研究沉默信息调节因子1(SIRT1)在合并2型糖尿病子宫内膜癌中的表达,及其与子宫内膜癌临床病理特征的相关性。方法:应用免疫组化法检测SIRT1在39例合并2型糖尿病子宫内膜癌、40例非合并糖尿病子宫内膜癌和12例合并糖尿病正常子宫内膜组织中的表达情况。分析SIRT1的表达与子宫内膜癌临床病理参数的关系及与p53、c-cerbB-2和Ki-67表达的相关性。结果:SIRT1在合并糖尿病子宫内膜癌组的阳性率为64.1%,高于合并糖尿病正常子宫内膜组的阳性率8.33%,差异有统计学意义(P=0.001),低于单纯子宫内膜癌组的阳性率85.0%,差异有统计学意义,P=0.033。39例合并糖尿病子宫内膜癌中,SIRT1与p53的表达呈正相关关系,r=0.360,P=0.024;与原癌基因c-erbB-2的表达呈正相关关系,r=0.775,P<0.01;与Ki-67的表达无明显相关性,r=0.022,P=0.896。SIRT1的表达与子宫内膜癌临床分期(P=0.023)和组织学分级(P=0.008)有关。结论:SIRT1表达与p53和c-erbB-2表达正相关,并参与糖代谢,糖尿病患者子宫内膜组织中SIRT1的表达增加,提示发生子宫内膜癌的风险增加。
Objective: To investigate the expression of SIRT1 in endometrial carcinoma with type 2 diabetes mellitus (T2DM) and its relationship with the clinicopathological features of endometrial carcinoma. Methods: Immunohistochemistry was used to detect the expression of SIRT1 in 39 cases of endometrial carcinoma with type 2 diabetes mellitus, 40 cases of non-diabetic endometrial carcinoma and 12 cases of normal endometrium with diabetes mellitus. To analyze the relationship between the expression of SIRT1 and the clinicopathological parameters of endometrial carcinoma and its correlation with the expression of p53, c-cerbB-2 and Ki-67. Results: The positive rate of SIRT1 in diabetic endometrial cancer group was 64.1%, which was higher than that in normal endometrium group with diabetes mellitus (8.33%), the difference was statistically significant (P = 0.001), lower than that in simple endometrium The positive rate of SIRT1 and p53 in cancer group was 85.0%, P = 0.033. There was a positive correlation between SIRT1 and p53 expression (P = 0.033), r = 0.360, P = 0.024 -erbB-2 expression was positively correlated, r = 0.775, P <0.01; and Ki-67 expression was not significantly correlated, r = 0.022, P = 0.896. The expression of SIRT1 was correlated with clinical stage (P = 0.023) and histological grade (P = 0.008) of endometrial carcinoma. CONCLUSIONS: SIRT1 expression is positively correlated with p53 and c-erbB-2 expression and is involved in glucose metabolism. The increased expression of SIRT1 in endometrial tissue suggests that the risk of endometrial cancer is increased.