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研究了庚型肝炎病毒E2 (HGVE2 )基因片段作为DNA疫苗的可行性。将来自于质粒pThioHis E2编码HGVE2的基因片段 (5 5 9bp)亚克隆到质粒pCMV S中 ,使之和HBsAg基因位于同一阅读框 ,形成重组质粒pCMV S E2。用纯化的质粒pCMV S E2DNA注射到昆明小鼠后腿四头肌中来免疫小鼠 ,同时用pCMV S作为对照。间隔 14天再加强一次免疫。在加强免疫后的第 8天眼眶取血。用E2 GST融合蛋白作为固定化抗原 ,通过ELISA检测受试小鼠的体液免疫应答。结果表明 ,用质粒pCMV S EDNA免疫的小鼠可以产生很强的体液免疫应答
The feasibility of using DNA fragment of hepatitis G virus E2 (HGVE2) as DNA vaccine was studied. The gene fragment (5 59 bp) encoding HGVE2 from plasmid pThioHis E2 was subcloned into plasmid pCMV S in the same reading frame as the HBsAg gene to form the recombinant plasmid pCMV S E2. Mice were immunized with the purified plasmid pCMV S-E2 DNA injected into the quadriceps muscle of Kunming mice, while pCMV S was used as a control. Reinforce once more after 14 days. Eighth day after booster immunization orbital blood. The humoral immune response of the test mice was tested by ELISA using the E2 GST fusion protein as an immobilized antigen. The results show that mice immunized with the plasmid pCMV S EDNA can produce a strong humoral immune response