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[目的]观察mi RNA-145对肺癌细胞系A549迁移、侵袭和克隆形成的影响,探讨其潜在的应用价值。[方法]采用mi RNA-145过表达的慢病毒载体上调A549细胞中mi RNA-145的表达量,Transwell迁移及侵袭实验分析mi RNA-145表达上调前后A549细胞的体外迁移、侵袭能力,平板克隆形成实验分析mi RNA-145表达上调前后A549细胞的体外增殖能力。[结果]RT-PCR结果表明,侵染了mi RNA-145过表达载体的A549细胞中mi RNA-145水平明显高于阴性对照组及空白对照组,差异有统计学意义(P<0.01);Transwell迁移和侵袭实验结果表明,过表达mi RNA-145的A549细胞过膜数少于阴性对照及空白对照组,差异有统计学意义(P<0.01);平板克隆形成实验结果表明,过表达mi RNA-145的A549细胞平板克隆形成能力明显降低,差异有统计学意义(P<0.01)。[结论]mi RNA-145的过表达能够抑制肺癌细胞系A549的迁移、侵袭和克隆形成,由此提示mi RNA-145有可能成为肺癌治疗的潜在靶点。
[Objective] To observe the effect of miRNA-145 on the migration, invasion and cloning of lung cancer cell line A549 and to explore its potential value. [Methods] The miRNA-145 expression in A549 cells was induced by lentiviral vector overexpression of mi RNA-145. Transwell migration and invasion assay were used to analyze the migration and invasion ability of A549 cells before and after miRNA-145 expression was up-regulated. To form an experimental analysis of miRNA-145 expression before and after A549 cell proliferation in vitro. [Results] RT-PCR results showed that the mi RNA-145 level in A549 cells infected with mi RNA-145 overexpression vector was significantly higher than that in negative control group and blank control group (P <0.01). The results of Transwell migration and invasion showed that the number of over-expressed mi RNA-145 in A549 cells was less than that in negative control group and blank control group (P <0.01). The results of plate clone formation assay showed that overexpression mi The colony-forming ability of A549 cells transfected with RNA-145 was significantly decreased (P <0.01). [Conclusion] Overexpression of mi RNA-145 can inhibit the migration, invasion and colony formation of lung cancer cell line A549, which indicates that miRNA-145 may be a potential target for the treatment of lung cancer.