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苯妥英是一种常甩的抗癫痫和抗心律失常药物,它的有效治疗血浓度一般为10～20μg/ml,超过此范围时,会产生中毒现象。由于个体的差异性,相同剂量而血浓度可不一样。为了确保用药安全,宜在用药期间,经常检测血中苯妥英的含量进行监护。 苯妥英在碱性高锰酸钾溶液中被氧化成二苯酮后,用环已烷提取,在247nm处测出吸收值后进行运算。其具体操作步骤是:取病人血清1.0(或1.5)ml,置于2ml pH6.8的磷酸盐缓冲液中,用二氯甲烷10ml提取二次,合并提取液,加入7N NaOH7ml,摇匀,分离出碱液,加热除去残留的二氯甲烷,然后加饱和KMnO_4溶液2ml,摇匀密塞,于80℃水浴加热15～25分钟,取出冷至室温,加入2.5ml环己烷提取,在247nm处测出提取液吸收值y,苯妥英浓度t在1～30μg/ml血清时,与y的回归方程为y=0.02061x+0.003163,r=0.9999,回收率为98.32％±0.58％,cu％为0.59％。
Phenytoin is a commonly used anti-epileptic and anti-arrhythmic drug, its effective therapeutic blood concentration is generally 10 ~ 20μg / ml, beyond this range, it will produce poisoning. Due to individual differences, the same dose and blood concentration can be different. In order to ensure the safety of medication, it is appropriate to monitor the content of phenytoin in blood regularly during medication. Phenytoin in alkaline solution of potassium permanganate was oxidized to benzophenone, with cyclohexane extraction, measured at 247nm absorbance calculation. The specific steps are: take the patient serum 1.0 (or 1.5) ml, placed in 2ml pH6.8 phosphate buffer, extracted with methylene chloride 10ml twice, the combined extract was added 7N NaOH7ml, shake, separate A lye, heated to remove residual methylene chloride, and then add saturated KMnO_4 solution 2ml, shaking the plug, at 80 ℃ water bath for 15 to 25 minutes, remove the cold to room temperature, was added 2.5ml cyclohexane extraction at 247nm The regression equation of y with phenytoin concentration t of 1 ~ 30μg / ml was y = 0.02061x + 0.003163, r = 0.9999, the recoveries were 98.32% ± 0.58% and cu% was 0.59 %.